Evidence map›Paper›PMID 41225027›Full record

ArticleScientific reports2025

Xiaoyao San against depression via glia-mediated neuroinflammation using network pharmacology and experimental validation.

Xin-Yi Liu, Yi-Zhi Yan, An-Jun Jiang, Wan-Ying He, Ying-Yan Fang, Chao-Hui Li, Man-Fei Deng, Si-Jie Tan, Peng Zeng

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. [Nan fang yi ke da xue xue bao = Journal of Southern Medical University
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Xin-Yi Liu *Department of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Yi-Zhi Yan *Department of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China.
An-Jun JiangDepartment of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Wan-Ying HeDepartment of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Ying-Yan FangHubei Key Laboratory for Kidney Disease Pathogenesis and Intervention, Hubei Polytechnic University School of Medicine, Huangshi, 435003, China.
Chao-Hui LiLogistics service center medical office, University of South China, Hengyang, 421001, China.
Man-Fei DengDepartment of Physiology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Si-Jie TanDepartment of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China. sjtan@usc.edu.cn.
Peng ZengDepartment of Histology and Embryology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, China. zengp@usc.edu.cn.

Funding

National Natural Science Foundation of China 82301506Open Fund Project of Hubei Key Laboratory for Kidney Disease Pathogenesis and Intervention 2023SB101Provincial Natural Science Foundation of Hunan 2023JJ40562Science and Technology Innovation Project for College Students 202510555083
6 · The paper itself

Abstract

Xiaoyao San (XYS) has been proven effective in treating depression, but its underlying mechanisms remain to be elucidated. This study aims to explore the key phytochemicals and mechanisms of the anti-depressant effects of XYS. We focus on the 16 blood compounds of XYS identified through the UPLC-MS/MS method. We also validated the results of the network pharmacology analysis using an lipopolysaccharide (LPS)-induced depression mouse model and the BV2 microglial cell line. In silico prediction, atractylenolide I, ferulic acid, kaempferol, quercetin, and vanillic acid exhibit good oral bioavailability, safety, and ADMET properties. The results of GSEA and GO enrichment analysis showed that neuroinflammation is the key mechanism of the anti-depressant effect of XYS. Kaempferol, quercetin, atractylenolide II, z-ligustilide, paeoniflorin, saikosaponin A, atractylenolide III and glycyrrhizic acid (GA) were identified as the main phytochemicals targeting neuroinflammation. XYS gavage treatment significantly ameliorated LPS-induced depressive-like behaviors in mice by reducing neuroinflammation (microglial activation) in the prefrontal cortex. The reduction of LPS-induced pro-inflammatory cytokine levels by GA was also validated in BV2 cells, and its mechanism is associated with JNK pathway. The phytochemicals of XYS exhibit favorable safety and pharmacological parameters, with targeting neuroinflammation being the key mechanism underlying its anti-depressant effects.

Indexed as

Antidepressive AgentsDepressionDrugs, Chinese HerbalNeuroinflammatory DiseasesAnimalsCell LineDisease Models, AnimalLipopolysaccharidesMaleMiceMicrogliaNetwork PharmacologyAntidepressive AgentsDrugs, Chinese HerbalLipopolysaccharidesxiaoyaosanDepressionLipopolysaccharideNetwork pharmacologyNeuroinflammationPhytochemicalsXiaoyao san

Identifiers

PMID41225027
PMCPMC12612169

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.