Evidence map›Paper›PMID 41224769›Full record

ArticleNature communications2025

Colistin resistance plasmids dually enhance bacterial virulence and antibiotic resistance via surface polysaccharide biosynthesis.

Eunbyeol Ahn, Jinshil Kim, Junyao Jiang, Joonbeom Kim, Artur Muszyński, Katarzyna Kasperkiewicz, Hojun Shin, Yingqi Cao, Yejin Soh, Youngjae Park and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Eunbyeol Ahn *Department of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.ORCID http://orcid.org/0009-0004-9926-5996
Jinshil Kim *Department of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Junyao Jiang *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Joonbeom KimDepartment of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.ORCID http://orcid.org/0009-0007-2119-0789
Artur MuszyńskiComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.ORCID http://orcid.org/0000-0003-3497-9977
Katarzyna KasperkiewiczInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Katowice, Poland.
Hojun ShinDepartment of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Yingqi CaoState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yejin SohDepartment of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Youngjae ParkDepartment of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Christian HeissComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Liyanage Devthilini Pasasum FernandoComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Parastoo AzadiComplex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Yang WangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Byeonghwa JeonEnvironmental Health Sciences, School of Public Health, University of Minnesota, St. Paul, MN, USA. bjeon@umn.edu.ORCID http://orcid.org/0000-0002-6665-5558
Sangryeol RyuDepartment of Food and Animal Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea. sangryu@snu.ac.kr.ORCID http://orcid.org/0000-0001-5812-3394

Funding

National Glycoscience Resource- CCRC Service and Training - NGlycoR@CCRCR24GM137782 · NIGMS · UNIVERSITY OF GEORGIA · PI Parastoo Azadi · 2020 to 2026
$5.2M
National Research Foundation of Korea (NRF) NRF-2023R1A2C1006359NIGMS NIH HHS R24 GM137782U.S. Department of Energy (DOE) DE-SC0015662U.S. Department of Health & Human Services | National Institutes of Health (NIH) R24GM137782
6 · The paper itself

Abstract

Plasmids carrying the mobilized colistin-resistance gene mcr-1 are prevalent among multidrug-resistant Gram-negative pathogens, yet their broad impact on bacterial physiology and virulence remains unclear. Here, we demonstrate that acquisition of an mcr-1 plasmid concurrently increases antimicrobial resistance and pathogenicity in Escherichia coli. On the same plasmid, the XRE-family transcriptional regulator EcaR cooperates with MCR-1 to activate the wec operon, driving biosynthesis of two surface polysaccharides: enterobacterial common antigen (ECA) and a high-molecular-weight O-chain. Expression of these surface polysaccharides increases bile resistance and virulence in a murine model and further elevates colistin resistance. MCR-1 enhances transcription of upstream genes in the wec operon, whereas EcaR directly activates an internal promoter (P

Indexed as

ColistinDrug Resistance, BacterialEscherichia coliPlasmidsPolysaccharides, BacterialAnimalsAnti-Bacterial AgentsEscherichia coli InfectionsEscherichia coli ProteinsGene Expression Regulation, BacterialMiceOperonVirulenceAnti-Bacterial AgentsColistinEscherichia coli ProteinsMCR-1 protein, E coliPolysaccharides, Bacterial

Identifiers

PMID41224769
PMCPMC12612032

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.