ArticleNature communications2025
FUS nanoclusters are a distinct state within the dilute phase.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- α-Synuclein Forms Distinct Micelle-Like Assemblies at Low Ionic Strengths.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Beyond equilibrium in biomolecular phase separation.Nature structural & molecular biology · 2026Article
- Current Challenges of Transcription Compartmentalization Research.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- The exchange dynamics of client molecules in biomolecular condensates.bioRxiv : the preprint server for biology · 2026Article
- Single-Molecule Dwell Times in Biomolecular Condensates.bioRxiv : the preprint server for biology · 2026Article
- Single-cluster pull-down assay for visualization and characterization of nanoclusters and condensates.STAR protocols · 2026Article
- Distinguishing near- versus off-critical phase behaviors of intrinsically disordered proteins.Reports on progress in physics. Physical Society (Great Britain) · 2026Article
- Oligomerization of PER2 as a dynamic mode of regulation of the mammalian circadian clock.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nuclear-import receptors remodel the dilute phase to suppress phase transitions of RNA-binding proteins with prion-like domains.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Liquid-liquid phase separation of Fused in sarcoma (FUS) has been extensively studied due to its critical role in various physiological and pathological processes. However, the mechanism of this process remains poorly understood. Here we show that by preparing pre-cleaved FUS, we enable kinetic measurement of phase separation from the onset. We identify a distinct state within the dilute phase using a combination of biophysical measurements including dynamic light scattering, fluorescence correlation spectroscopy, and an optimized single-cluster fluorescence imaging assay. This state is termed nanoclusters. They are hundreds of nanometers in diameter and exhibit instantaneous formation far below the saturation concentration, sharply arrested coarsening, rapid mixing, high exchange rate, and resistance to hexanediol-induced dissolution. This state exhibits properties that distinguish it from condensates. Our findings define the nanocluster as a prominent intermediate in the FUS phase separation pathway. This understanding paves the way for further experiments and theories to explore the molecular mechanisms underlying FUS and other phase-separating proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.