Evidence map›Paper›PMID 41224186›Full record

ArticleExperimental eye research2026

Inhibition of pathological angiogenesis in oxygen-induced retinopathy by arginine depletion with ADI-PEG20.

Porsche V Sandow, Syed A H Zaidi, Mai Yamamoto, Zhimin Xu, Ruth B Caldwell, R William Caldwell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Porsche V SandowDepartment of Pharmacology and Toxicology, Augusta University, Augusta, GA, 30912, USA; Culver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA.
Syed A H ZaidiCulver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA; Vascular Biology Center, Augusta University, Augusta, GA, 30912, USA; Department of Medicine, Augusta University, Augusta, GA, 30912, USA.
Mai YamamotoCulver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA; Vascular Biology Center, Augusta University, Augusta, GA, 30912, USA.
Zhimin XuCulver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA; Vascular Biology Center, Augusta University, Augusta, GA, 30912, USA.
Ruth B CaldwellCulver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA; Vascular Biology Center, Augusta University, Augusta, GA, 30912, USA; Department of Cellular Biology and Anatomy at the Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.
R William CaldwellDepartment of Pharmacology and Toxicology, Augusta University, Augusta, GA, 30912, USA; Culver Vision Discovery Institute, Augusta University, Augusta, GA, 30912, USA. Electronic address: wcaldwel@augusta.edu.

Funding

Cellular Mechanisms of Retinopathy: Role of ArginaseR01EY011766 · NEI · MEDICAL COLLEGE OF GEORGIA (MCG) · PI CALDWELL, ROBERT WILLIAM, CALDWELL, RUTH B · 1998 to 2021
$6.7M
Module 3: Gene Expression/ProteomicsP30EY031631 · NEI · AUGUSTA UNIVERSITY · PI Xingjun Fan · 2020 to 2026
$3.6M
Myeloid ACAT1 in ischemic retinopathyR01EY035683 · NEI · AUGUSTA UNIVERSITY · PI Ruth B Caldwell, Modesto Antonio Rojas · 2024 to 2026
$1.2M
NEI NIH HHS P30 EY031631NEI NIH HHS R01 EY011766NEI NIH HHS R01 EY035683
6 · The paper itself

Abstract

Pathological retinal neovascularization (RNV) is vision-threatening. We tested the efficacy of a stable form of arginine deiminase (ADI-PEG20), which has antioxidant and anti-inflammatory actions, as a novel therapy for pathological RNV using a model of oxygen-induced retinopathy (OIR). Neonatal C57BL/6J mice and nursing dams were maintained in 75 % oxygen or room air as control from postnatal day 7 (P7) to P12. Mice were then treated with intravitreal injections of ADI-PEG20 or control PEG20. On P17, eyes were collected for analysis of avascular area, retinal neovascularization (RNV), and vascular tip cell characteristics. Other pups treated with ADI-PEG20 or PEG20 were maintained through P30 and tested for visual acuity, neuronal function, and vascular tortuosity. Human retinal microvascular endothelial cell (HREC) spheroids were also examined for effects of ADI-PEG20 on sprouting. Treatment of OIR mice with ADI-PEG20 significantly reduced both avascular area and RNV, increased vascular tip cell formation, and improved their morphology, as compared to PEG20-treated controls. Further, tip cells in the sprouts in ADI-PEG20 treated spheroids had fewer filopodia-like extensions, in response to VEGF compared to control spheroids. Mice treated with ADI-PEG20 showed improved visual acuity and decreased vascular tortuosity compared to PEG20 controls. Retinal electroretinography revealed preservation of scotopic-b wave amplitude in ADI-PEG20 treated mice. ADI-PEG20 did not alter photopic-b wave or a wave amplitude. Hence, intravitreal injection of ADI-PEG20 offers a novel strategy for limiting pathological angiogenesis, promoting vascular repair, and preserving visual function in conditions of ischemic retinopathy.

Indexed as

ArginineHydrolasesRetinal NeovascularizationRetinopathy of PrematurityAnimalsAnimals, NewbornDisease Models, AnimalElectroretinographyFemaleHumansIntravitreal InjectionsMiceMice, Inbred C57BLOxygenRetinal VesselsVisual AcuityArginineHydrolasesOxygenArginine deiminaseL-arginine depletionOxygen-induced retinopathyPathological angiogenesisRetinal neovascularizationTip cells

Identifiers

PMID41224186
PMCPMC13107968

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.