ArticleNucleic acids research2025
GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Losing genes, gaining edits: how relaxed selection and inverted repeat expansion shape RNA editing in Schizaeaceae plastomes.The Plant journal : for cell and molecular biology · 2026Article
- Structures of pentatricopeptide repeat proteins.Acta crystallographica. Section F, Structural biology communications · 2026Review
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Authors and funding
9 authors.
Funding
Abstract
Pentatricopeptide repeat (PPR) proteins are eukaryotic RNA-binding proteins with multiple roles in mitochondrial and chloroplast transcript processing. PPR proteins are naturally modular and hold great potential for development into tools for RNA processing or controlling RNA folding or expression. However, construction of synthetic PPR (sPPR) proteins is challenging due to their highly repetitive sequences. Here, we present the GRASP kit for assembly of sPPR proteins. Utilizing the S-variant of PPR motifs, we designed a library of 42 plasmids which can be combined to assemble sPPR proteins with 9, 14, or 19 motifs to target any RNA sequence of the same length. The GRASP kit enables rapid design and construction of PPR proteins of any desired specificity and is compatible with the MoClo assembly standard. To demonstrate the capabilities of GRASP, we assembled a sPPR-RNA-editing protein and variants with altered sequence specificity. We tested the functionality of 31 sPPR protein variants against a set of 46 RNA targets and used RNA sequencing to determine levels of RNA editing. The variations in editing provide a wealth of insights into PPR-RNA interactions. The GRASP kit provides a foundation for further development of sPPR protein technologies.
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Registered trials
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