Evidence map›Paper›PMID 41221789›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

A Dynamic Gate Enables Regioselective Hydroxylation of Free Arginine by a Non-Canonical Heme Enzyme.

Yuan Sun, Chao Dou, Weizhu Yan, Pengpeng Chen, Lu Zhang, Dan Zhou, Yanhui Zheng, Zhaolin Long, Shoujie Li, Xiaoqing Xu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yuan SunDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0009-0004-8915-0909
Chao DouAntibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu, 610106, China.ORCID https://orcid.org/0000-0003-0626-240X
Weizhu YanDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0000-0001-8648-1295
Pengpeng ChenDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Lu ZhangMetabolomics and Proteomics Technology Platform of Core Facilities, West China Hospital, Sichuan University, Chengdu, 610041, China.
Dan ZhouDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Yanhui ZhengDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0000-0003-4852-4252
Zhaolin LongDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Shoujie LiDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Xiaoqing XuDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Qiuxia HuangDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.
Xiaofeng ZhuDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0000-0002-1248-4247
Wei ChengDepartment of Pulmonary and Critical Care Medicine, Respiratory Infection and Intervention Laboratory of Frontiers Science Center for Disease-related Molecular Network, and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, 610041, China.ORCID https://orcid.org/0000-0002-7820-9774

Funding

China Postdoctoral Science Foundation 2022M722281China Postdoctoral Science Foundation 2024M752200Key Science and Technology Research Projects in Key Areas of the Corps 2023AB053National Natural Science Foundation of China 32200114National Science Fund for Distinguished Young Scholars 32225001Natural Science Foundation of Sichuan Province of China 2023NSFSC1197The 1.3.5 Project for Disciplines Excellence of West China Hospital, Sichuan University ZYGD23018
6 · The paper itself

Abstract

The YqcI/YcgG family of heme-dependent enzymes catalyzes guanidine N-H hydroxylation, a critical yet enigmatic step in bioactive natural product biosynthesis. Here, this mechanistic puzzle is resolved through high-resolution structural snapshots of AglA, a prototypical YqcI/YcgG member, revealing a non-canonical heme-binding "sandwich" fold. A dynamic regiochemical gating mechanism is uncovered: substrate-induced remodeling of loop L2 and key residues (Phe152, Arg179, Phe182) spatially constrains the guanidine group of aminomethylphosphonate-linked arginine (AMPn-Arg), enforcing exclusive internal N

Indexed as

ArginineBacterial ProteinsHemeHydroxylationMolecular Dynamics SimulationArginineBacterial ProteinsHemecrystal structuredynamic regiochemical gating mechanismheme‐dependent hydroxylaseself‐sufficient artificial chimeric enzymeYqcI/YcgG family enzymes

Identifiers

PMID41221789
PMCPMC12866846

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.