Evidence map›Paper›PMID 41221629›Full record

ArticleEmerging microbes & infections2025

Development of an over-gap droplet digital PCR assay and evaluation of serum HBcrAg/qAnti-HBc as noninvasive markers for intrahepatic replication-competent HBV DNA in chronic hepatitis B patients with HBsAg loss.

Guangxin Yu, Yan Liu, He Zhang, Mingwei Li, Le Li, Bei Jiang, Dandan Liang, Xiaoyong Li, Minggui Lin, Hongxing Han and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Emerging microbes & infections, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Guangxin YuDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
Yan LiuSenior Department of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, People's Republic of China.
He ZhangDepartment of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Mingwei LiMedical Affairs Department, The Fourth Hospital of Shijiazhuang, Shijiazhuang, People's Republic of China.
Le LiSenior Department of Infectious Diseases, the Fifth Medical Center of Chinese PLA General Hospital, National Clinical Research Center for Infectious Diseases, Beijing, People's Republic of China.
Bei JiangDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
Dandan LiangXiamen Amoytop Biotech Co., Ltd., Xiamen, People's Republic of China.
Xiaoyong LiDepartment of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Minggui LinDepartment of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Hongxing HanDepartment of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Jiuxin QuDepartment of Clinical Laboratory, Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Diseases, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Fengmin LuDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.ORCID 0000-0002-1832-3209
Hao LiaoDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study assessed serum hepatitis B core-related antigen (HBcrAg) and antibodies to hepatitis B core antigen (qAnti-HBc) as noninvasive biomarkers for detecting intrahepatic replication-competent HBV DNA (repDNA) in chronic hepatitis B (CHB) patients who had lost HBsAg. The goal was to identify alternatives to liver biopsy for diagnosing residual HBV activity after HBsAg loss. A novel over-gap droplet digital PCR (ddPCR) assay was developed for sensitive intrahepatic repDNA detection. Over-gap ddPCR detected repDNA in 88.24% (135/153) of patients, which was a significantly greater percentage than traditional nested PCR (75.16%, 115/153;

Indexed as

DNA, ViralHepatitis B, ChronicHepatitis B Core AntigensHepatitis B Surface AntigensHepatitis B virusPolymerase Chain ReactionAdultAgedBiomarkersFemaleHepatitis B AntibodiesHumansLiverMaleMiddle AgedSensitivity and SpecificityBiomarkersDNA, ViralHepatitis B AntibodiesHepatitis B Core AntigensHepatitis B Surface Antigensanti-HBcfunctional cureHBsAg lossHBV core-related antigenReplication-competent HBV DNA

Identifiers

PMID41221629
PMCPMC12667311

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.