Evidence map›Paper›PMID 41221025›Full record

ArticleBiology methods & protocols2025

ThermoPlex: an automated design tool for target-specific multiplex PCR primers based on DNA thermodynamics.

Altair Agmata, Kevin Labrador, Joseph Dominic Palermo, Maria Josefa Pante

Abstract read
In one paragraph

Article in Biology methods & protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Altair AgmataDepartment of Chemical Engineering, University of the Philippines, Diliman, Quezon City 1101, Philippines.ORCID https://orcid.org/0000-0002-7560-6037
Kevin LabradorThe Marine Science Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines.
Joseph Dominic PalermoThe Marine Science Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines.
Maria Josefa PanteThe Marine Science Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiplex PCR-based assays are indispensable platforms for rapid and cost-effective DNA-based multi-target detection. The success of such an assay highly depends on the accurate design of oligonucleotide primers, arguably its most vital component. In this study, the

Indexed as

DNA ThermodynamicsMultiplex PCRPrimer DesignTarget-specific PCR

Identifiers

PMID41221025
PMCPMC12598146

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.