ArticleJDS communications2025
Comparison of 4 different RNA sources from lactating dairy cows to assess the mammary transcript abundance.
Article in JDS communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Insulin sensitivity and mitochondrial homeostasis connect lactation to maternal metabolic health.The Journal of clinical investigation · 2026Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noninvasive sampling of milk fat for isolation of RNA is an alternative technique to assess the mammary transcriptome. However, contamination of RNA from milk fat with nonmammary sources of RNA may complicate the interpretation of results. We measured transcript abundance of 8 genes in RNA from milk fat, milk somatic cells, mammary tissue, and blood leukocytes to determine how the transcript abundance of selected genes compares between RNA sources. Samples from 8 healthy cows were harvested immediately after slaughter. Blood was collected during exsanguination. Milk and mammary tissue were collected from the same mammary gland. Mammary tissue was frozen immediately, and fluids were centrifuged to collect the buffy coat from blood, and the fat layer and cell pellet from milk. The RNA isolated from all tissue sources was prepared for reverse-transcription quantitative PCR. Relative transcript abundance for each gene was determined by normalizing the abundance of the target gene against the abundance of reference genes (
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Registered trials
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