Evidence map›Paper›PMID 41220748›Full record

ArticleJournal of gastrointestinal oncology2025

YTHDF1 regulates YTHDF2 stability via m6A-dependent mechanisms in hepatocellular carcinoma: insights from

Yue-Fan Wang, Zeng-Han Wang, Zhi-Chao Zhang, Jian Tan, Zhi-Xuan Li, Hao-Zan Yin, Xian-Jie Piao, Zhi-Hui Dai, Chen-Yang Mu, Si-Jie Wang and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of gastrointestinal oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Ubiquitination and NOncology letters · 2026
    Review
  2. mArXiv · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yue-Fan Wang *The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.ORCID https://orcid.org/0000-0001-5580-9198
Zeng-Han Wang *The Department of Medical Genetics, Naval Medical University, Shanghai, China.
Zhi-Chao Zhang *The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Jian TanThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Zhi-Xuan LiTranslational Medicine Research Center, Medical Innovation Research Division, and Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.
Hao-Zan YinThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Xian-Jie PiaoThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Zhi-Hui DaiThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Chen-Yang MuThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Si-Jie WangThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Qian-Jia LiuThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Jia-Cheng ZhangThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Fu-Chen LiuThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Wei DongThe Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Zheng-Guang WangThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Sheng-Xian YuanThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.
Fu YangThe Department of Medical Genetics, Naval Medical University, Shanghai, China.
Hui LiuThe Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval Medical University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Hepatocellular carcinoma (HCC) maintains a significant mortality burden, primarily attributable to the lack of validated prognostic molecular signatures. While N6-methyladenosine (m6A) reader proteins, especially YT521-B homology domain family proteins (YTHDFs), show emerging relevance in oncogenesis, their regulatory interplay and clinical utility as combinatorial biomarkers remain unclear. Our study aimed to explore the regulatory network and clinical relevance of YTHDF paralogs in HCC. Methods: Spatial co-expression patterns were analyzed via immunohistochemistry and spatial transcriptomics in HCC tissues. Protein/RNA levels of YTHDFs were quantified by western blot and quantitative real-time polymerase chain reaction (PCR). RNA immunoprecipitation (RIP), enhanced crosslinking and immunoprecipitation (e-CLIP) sequencing, dual fluorescence reporter assay, and RNA stability experiments were conducted to verify underlying regulatory mechanisms. Univariate and multivariate Cox regression analysis and Kaplan-Meier survival analysis were utilized to estimate the potential clinical significances. Results: YTHDF1 overexpression correlated with poor prognosis and emerged as the strongest prognostic marker among the YTHDF paralogs. YTHDF1 could bind to the messenger RNA (mRNA) of YTHDF2 and YTHDF3 at specific m6A sites. However, Conclusions: YTHDF1 drives HCC progression through m6A-mediated stabilization of YTHDF2, revealing their functional synergy. Their combined spatial and expression profiles offer a superior prognostic biomarker, suggesting novel therapeutic targets.

Indexed as

correlationshepatocellular carcinoma (HCC)N6-methyladenosine reader (m6A reader)prognosisYT521-B homology domain family protein paralogs (YTHDF paralogs)

Identifiers

PMID41220748
PMCPMC12598331

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.