Alzheimer's disease PSEN-2 N141I mutation reveals altered and shear-sensitive brain endothelial cell-like phenotype in human iPSC-derived models.

Lily E Takeuchi, Jennifer Lam, Craig A Simmons

Abstract read

In one paragraph

Article in Acta neuropathologica communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lily E TakeuchiInstitute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada. lily.takeuchi@mail.utoronto.ca.ORCID 0000-0002-9527-8042

Jennifer LamInstitute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.ORCID 0009-0004-5490-9161

Craig A SimmonsInstitute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada. c.simmons@utoronto.ca.ORCID 0000-0001-7729-1772

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2022-04375

6 · The paper itself

Abstract

Drug discovery efforts in neurological diseases, such as Alzheimer's disease (AD), have had particularly poor outcomes due to the lack of models that recapitulate drug interactions at the cerebral vasculature. There is an unmet need to develop physiologically relevant models to study the impacts of blood flow-induced shear stress. In this work, we use a microfluidic platform to model the cerebral vasculature in AD using patient-derived brain endothelial-like cells (BECs). Induced pluripotent stem cells derived from a patient with familial AD (PSEN-2 N141I) and an unaffected control line were differentiated into BECs (AD2-BEC and fControl-BEC, respectively). BECs were exposed to static conditions or 12 dynes/cm

Indexed as

Alzheimer DiseaseBrainEndothelial CellsInduced Pluripotent Stem CellsPresenilin-2ATP Binding Cassette Transporter, Subfamily G, Member 2HumansMonocytesMutationPhenotypeStress, MechanicalATP Binding Cassette Transporter, Subfamily G, Member 2Presenilin-2PSEN2 protein, humanAlzheimer’s diseaseBlood-brain barrierBrain endothelial cellsHypoperfusionMicrofluidicsStem cells

Identifiers

PMID41219801

DOI10.1186/s40478-025-02152-3

PMCPMC12607174

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.