ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2025
Identification of cDNA-AFLP fragments associated with the B chromosome from different developmental stages of maize anthers.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
B chromosomes are nonessential chromosomes and are considered genetically inert. However, the maize B chromosome possesses several genetic properties during male gamete development in anthers. To determine whether the transcriptome of maize anthers is influenced by the B chromosome, we established the correlation between anther length and developmental stage in the maize B73 inbred line and applied complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) analysis to microsporogenetic (MS) anthers, microgametogenetic (MG) anthers and mature pollen (MP) of B73 plants carrying zero (0B) and two B chromosomes (2B). The results revealed that 6.4%, 7.4% and 25.1% of 0B-extra or 2B-extra cDNA-AFLP fragments from MS anthers, MG anthers and MP, respectively, are present between B73 + 0B and B73 + 2B. Twelve out of the 13 2B-extra cDNA-AFLP fragments from MS anthers and MP are A chromosome-located sequences and are transcribed in the presence of the B chromosome. Some of these sequences are functional genes. The remaining 2B-extra cDNA-AFLP fragment isolated from MS anthers is a novel B chromosome-specific repeat, pMS4. On the basis of three B-10L translocations and fluorescence in situ hybridization, pMS4 was mapped to the short arm and the junction between the centromeric knob and the proximal euchromatin of the B chromosome. Reverse transcriptase PCR revealed that pMS4 is transcribed in a B chromosome dosage-dependent and tissue-specific manner. Taken together, our data suggest that the maize B chromosome can transcribe and influence the transcription of A chromosome-located genes in anthers at different developmental stages.
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