Evidence map›Paper›PMID 41219408›Full record

ArticleScientific reports2025

Novel proof of concept assay for rapid, simplified non-invasive fetal RhD screening.

Jean Gekas, Suvi Parviainen, Lawrence Prensky, Audrey Bélanger, Marc-André Rodrigue, Marie-Line Dubois, Capucine Gekas, Ville Veikkolainen

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jean GekasDepartment of Medical Genetics, Quebec University Mother and Child Center, Laval Medical University, Quebec City, QC, Canada.
Suvi ParviainenRevvity Inc, Turku, Finland.
Lawrence PrenskyRevvity Inc, 77 4th Ave, Waltham, MA, 02451, USA. lawrence.prensky@revvity.com.ORCID http://orcid.org/0000-0003-4427-9122
Audrey BélangerPrenato clinics, Quebec City, Canada.
Marc-André RodrigueDepartment of Medical Genetics, Quebec University Mother and Child Center, Laval Medical University, Quebec City, QC, Canada.
Marie-Line DuboisLaval Medical University, Quebec City, QC, Canada.
Capucine GekasSainte-Anne College, Lachine, QC, Canada.
Ville VeikkolainenRevvity Inc, Turku, Finland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The RhD antigen is a key component of the Rh blood group system and is highly immunogenic, posing risks for RhD-negative pregnant women. While anti-D prophylaxis prevents maternal alloimmunization, concerns regarding availability, overuse, and universal effectiveness persist. Recent advances in non-invasive fetal RHD genotyping have shown potential to reduce unnecessary interventions. This study presents a new non-invasive fetal RhD screening method using cell-free DNA (cfDNA) and dry chemistry quantitative polymerase chain reaction (qPCR) with fluorescence measurements of three RHD exons, aimed at enhancing the accuracy and efficiency of RHD genotyping and improving clinical decision-making. We evaluated this new method using cfDNA extracted from plasma samples of 28 RhD-negative women carrying singleton pregnancies. The 20µL cfDNA samples correctly identified all 18 RhD-positive pregnancies and 9/10 RhD-negative pregnancy samples. Our study provides proof of concept that the cfDNA and dry chemistry qPCR method for fetal RHD genotyping is feasible, as it achieved 100% sensitivity. Despite the small sample size, these findings support the use of RHD genotyping as a precise and effective alternative or complement to anti-D prophylaxis, offering improved risk management and care for pregnant women worldwide.

Indexed as

Prenatal DiagnosisRh-Hr Blood-Group SystemAdultCell-Free Nucleic AcidsFemaleGenotypeGenotyping TechniquesHumansPregnancyProof of Concept StudyCell-Free Nucleic AcidsRh-Hr Blood-Group SystemRho(D) antigenCell-free DNADry chemistry qPCRPregnancy managementPrenatal screeningRhDRHD genotyping

Identifiers

PMID41219408
PMCPMC12606211

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.