Evidence map›Paper›PMID 41218610›Full record

ArticleCell reports2025

KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.

Jiahang Miao, Masatoshi Hara, Kuan-Chung Su, Heather R Keys, Weixia Kong, Yusuke Takenoshita, Iain M Cheeseman, Tatsuo Fukagawa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jiahang MiaoGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka 565-0871, Japan.
Masatoshi HaraGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka 565-0871, Japan; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: masahara@g.ecc.u-tokyo.ac.jp.
Kuan-Chung SuWhitehead Institute for Biomedical Research, 455 Main Street, Cambridge, MA 02142, USA.
Heather R KeysWhitehead Institute for Biomedical Research, 455 Main Street, Cambridge, MA 02142, USA.
Weixia KongGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka 565-0871, Japan.
Yusuke TakenoshitaGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka 565-0871, Japan.
Iain M CheesemanWhitehead Institute for Biomedical Research, 455 Main Street, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
Tatsuo FukagawaGraduate School of Frontier Biosciences, The University of Osaka, Suita, Osaka 565-0871, Japan. Electronic address: fukagawa.tatsuo.fbs@osaka-u.ac.jp.

Funding

Molecular Analysis of Kinetochore FunctionR35GM126930 · NIGMS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Iain McPherson Cheeseman · 2018 to 2026
$7.0M
NIGMS NIH HHS R35 GM126930
6 · The paper itself

Abstract

Chromosome congression is a key process that acts to align chromosomes at the spindle equator via kinetochore-microtubule interactions, with defects in chromosome alignment leading to chromosomal instability. However, defining the mechanisms that underlie chromosome congression is limited due to the multiple factors that act in parallel to regulate chromosome movement. Here, we conducted a genome-wide Cas9-based functional genetics screen using a hypomorphic CENP-C mutant that affects its kinetochore interactions. Our analysis identified KIF18A, whose knockout resulted in synthetic lethality with the CENP-C mutant. Further analysis revealed that the synthetic defect was due to a reduction in CENP-E function in the CENP-C mutant. Our work suggests that KIF18A promotes chromosome alignment in cooperation with CENP-E downstream of CENP-C during early prometaphase. Thus, our analysis enables us to dissect parallel molecular mechanisms for chromosome congression and identify sensitivities and biomarkers that might guide anti-KIF18A chemotherapeutics.

Indexed as

Chromosomal Proteins, Non-HistoneChromosome SegregationKinesinsHeLa CellsHumansKinetochorescentromere protein EChromosomal Proteins, Non-HistoneKIF18A protein, humanKinesinsCENP-CCENP-ECP: Molecular biologyKIF18AkinetochoreKMN network

Identifiers

PMID41218610
PMCPMC12834474

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.