Evidence map›Paper›PMID 41218605›Full record

ArticleCell reports methods2025

High-speed neural imaging with multiplexed miniaturized two-photon microscopy.

Zixiao Zhang, Shing-Jiuan Liu, Ben Mattison, Jessie Muir, Noah Spurr, Christina K Kim, Weijian Yang

Abstract read
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zixiao ZhangDepartment of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.
Shing-Jiuan LiuDepartment of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.
Ben MattisonDepartment of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA; Biomedical Engineering Graduate Group, University of California, Davis, Davis, CA 95616, USA.
Jessie MuirCenter for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Department of Neurology, School of Medicine, University of California, Davis, Sacramento, CA 95817, USA.
Noah SpurrDepartment of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.
Christina K KimCenter for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Department of Neurology, School of Medicine, University of California, Davis, Sacramento, CA 95817, USA.
Weijian YangDepartment of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA; Biomedical Engineering Graduate Group, University of California, Davis, Davis, CA 95616, USA. Electronic address: wejyang@ucdavis.edu.

Funding

Activity-dependent probes for spatially-defined proteomicsDP2MH136588 · NIMH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Christina Kim · 2023 to 2026
$2.4M
Large-field-of-view high-throughput two-photon endoscope to image neuronal activityR01NS118289 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI YANG, WEIJIAN · 2020 to 2020
$1.4M
Simultaneous two-photon imaging and two-photon manipulation of neural activity in freely behaving miceR21EB035306 · NIBIB · UNIVERSITY OF CALIFORNIA AT DAVIS · PI YANG, WEIJIAN · 2024 to 2025
$398k
NIBIB NIH HHS R21 EB035306NIMH NIH HHS DP2 MH136588NINDS NIH HHS R01 NS118289
6 · The paper itself

Abstract

Head-mounted miniaturized two-photon microscopes enable cellular-resolution recording of neural activity deep in the mouse brain during unrestrained behavior. Two-photon microscopy, however, is traditionally limited in frame rate by the necessity of scanning the excitation beam over a large field-of-view (FOV). Here, we present two types of multiplexed miniaturized two-photon microscopes (M-MINI2Ps) that preserve spatial resolution while increasing frame rate by simultaneously imaging two FOVs and demixing them temporally or computationally. We demonstrate large-scale (500 × 500 μm

Indexed as

Microscopy, Fluorescence, MultiphotonMiniaturizationNeuronsAnimalsCalciumMaleMiceMice, Inbred C57BLPrefrontal CortexVisual CortexCalciumbeam multiplexingcalcium imagingCP: imagingfreely behavingfreely movingminiaturized two-photon microscopeM-MINI2Ptwo-photon miniscopevoltage imaging

Identifiers

PMID41218605
PMCPMC12859475

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.