Evidence map›Paper›PMID 41217718›Full record

ArticleProbiotics and antimicrobial proteins2026

Probiotic Apilactobacillus Kunkeei EABW06 from Beeswax Microbiota: A Candidate for Functional Dairy Foods.

Mariam Hassan, Yasser Essam Elenany, Ashwak Abdel-Moneim Hassan, Noha A Ahmed

Abstract read
In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mariam HassanDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, 11562, Egypt. mariam.hassan@pharma.cu.edu.eg.
Yasser Essam ElenanyDepartment of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt.
Ashwak Abdel-Moneim HassanDepartment of Dairy Science, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt.
Noha A AhmedDepartment of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, Cairo, 11562, Egypt. noha.adel@pharma.cu.edu.eg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Apilactobacillus kunkeei, a fructophilic lactic acid bacterium (FLAB) associated with the honeybee microbiota, has recently gained attention as an unconventional probiotic source. This study evaluated the probiotic, safety, and technological properties of A. kunkeei EABW06, isolated from beeswax, to assess its potential for human and biotechnological applications. A. kunkeei EABW06 demonstrated gastrointestinal resilience, with > 96% survival at pH 3.0 and tolerance to 0.3-0.7% bile salts. It exhibited broad-spectrum antimicrobial activity against Escherichia coli O157:H7, Salmonella typhi, and Clostridioides difficile. Additionally, A. kunkeei EABW06 inhibited fungal pathogens including Aspergillus fumigatus, Aspergillus flavus, and Candida albicans. HPLC analysis revealed acetic, lactic, butyric, and propionic acids as dominant metabolites, correlating with its antimicrobial effects. Safety assessments confirmed no hemolysis, non-cytotoxicity to Caco-2 cells, and sensitivity to clinically relevant antibiotics. In milk fermentation trials, A. kunkeei EABW06 reduced pH to 4.5 ± 0.1, produced 0.98 ± 0.03% lactic acid (titratable acidity), and exhibited proteolytic activity (0.384 ± 0.002) measured by spectrophotometric quantification. In conclusion, A. kunkeei EABW06 is a safe, robust probiotic candidate with potential applications in biopreservation and dairy biotechnology.

Indexed as

Functional FoodProbioticsWaxesAnimalsBeesCaco-2 CellsFermentationHumansMicrobiotaMilkbeeswaxWaxesAcid and bile toleranceAntifungalAntimicrobial activityCaco-2 cytotoxicityDairy fermentationFood safety

Identifiers

PMID41217718
PMCPMC13342038

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.