Evidence map›Paper›PMID 41217625›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Biometric Quantification of Keratinocyte Turnover Using Bioengineered Human Skin Equivalents.

Georgia Abraham, Julia Maczkowska, Rachel Gilmore, Nicole Barrett, Jessica Simpson, Paola De Los Santos Gomez, Kirsty Goncalves, Victoria Maltman, Stefan Przyborski

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Georgia Abraham *Department of Biosciences, Durham University, Durham, UK.
Julia Maczkowska *Department of Biosciences, Durham University, Durham, UK.
Rachel GilmoreDepartment of Biosciences, Durham University, Durham, UK.
Nicole BarrettDepartment of Biosciences, Durham University, Durham, UK.
Jessica SimpsonDepartment of Biosciences, Durham University, Durham, UK.
Paola De Los Santos GomezDepartment of Biosciences, Durham University, Durham, UK.
Kirsty GoncalvesDepartment of Biosciences, Durham University, Durham, UK.
Victoria MaltmanDepartment of Biosciences, Durham University, Durham, UK.
Stefan PrzyborskiDepartment of Biosciences, Durham University, Durham, UK. stefan.przyborski@durham.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Effective epidermal cell turnover is essential for the formation and maintenance of the epidermis. Dysregulation of keratinocyte turnover impedes skin barrier integrity and contributes to disease pathophysiology. Full-thickness human skin equivalents (FT-HSE) offer a physiologically relevant in vitro platform for studying skin biology, providing valuable insights into the epidermal response to both endogenous and exogenous stimuli. This chapter describes immunofluorescent staining of Ki67 and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay for assessing cellular proliferation and apoptosis, thereby providing insights into keratinocyte turnover within FT-HSEs.

Indexed as

KeratinocytesSkinApoptosisCell ProliferationCells, CulturedEpidermisFluorescent Antibody TechniqueHumansIn Situ Nick-End LabelingKi-67 AntigenKi-67 AntigenApoptosisHuman skin equivalentsImmunofluorescenceKeratinocyteKi67ProliferationTUNEL AssayTurnover

Identifiers

PMID41217625

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.