ArticleWorld journal of microbiology & biotechnology2025
Monomeric Glycine oxidase from Azotobacter vinelandii for Glycine biosensing.
Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Glycine level is an indicator of diseases related to the central nervous system and metabolic disorders such as diabetes and obesity. It is, therefore, relevant to have tools that can detect glycine at physiological concentrations. Biosensors are a reliable alternative to chemical methods for glycine detection, with low cost and high response speed. In the case of glycine detection, a glycine oxidase (GO) can be used as the molecular recognition element in a biosensor. The GO from Azotobacter vinelandii is an uncharacterized enzyme with high sequence identity to the only monomeric FAD-dependent GO from P. putida KT2440. The monomeric state is favorable for oriented immobilization of active sites in a biosensor, which is attractive for future applications. This work characterized the GO from A. vinelandii, and a second-generation glycine amperometric biosensor was assembled. The enzyme was recombinantly expressed and purified. The oligomeric state of the enzyme was also confirmed as the monomer. Therefore, a substrate inhibition behavior was detected in the enzyme kinetics experiments with parameters K
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