Improved biosynthesis of lactulose by a novel thermostable cellobiose-2-epimerase generated through semi-rational mutagenesis.

Chenggang Zhong, Weijian Ding, Haoran Lin, Jin Huang

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Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Chenggang ZhongCollege of Pharmaceutical Science, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, 310014, P. R. China.

Weijian DingCollege of Pharmaceutical Science, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, 310014, P. R. China.

Haoran LinCollege of Pharmaceutical Science, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, 310014, P. R. China.

Jin HuangCollege of Pharmaceutical Science, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, 310014, P. R. China. huangjin979@zjut.edu.cn.ORCID http://orcid.org/0000-0002-9356-0589

Funding

Guiyuan foundation of Zhejiang university of Technology, China 20120401Hua-sen foundation of Zhejiang university of Technology, China KYY-HX-20230315

6 · The paper itself

Abstract

Cellobiose-2-epimerase is considered an ideal biocatalyst for the strictly thermodynamic synthesis of lactulose. However, its poor thermolability limits its industrial application. Herein, we successfully developed a novel thermostable cellobiose-2-epimerase for improving lactulose biosynthesis from lactose. The A242L mutant, derived from Dictyoglomus thermophilum (DiCE), was generated using semi-rational mutagenesis after computer-aided design via the FireProt web server. After systematic enzymatic characterization and optimization, A242L mutant exhibited a 123.5% T

Indexed as

Carbohydrate EpimerasesLactuloseBacterial ProteinsCellobioseEnzyme StabilityEscherichia coliHydrogen-Ion ConcentrationKineticsLactoseMolecular Docking SimulationMolecular Dynamics SimulationMutagenesisRacemases and EpimerasesTemperatureBacterial ProteinsCarbohydrate EpimerasesCellobioseLactoseLactuloseRacemases and EpimerasesCellobiose-2-epimeraseFireProtLactuloseSemi-rational mutagenesisThermostability

Identifiers

PMID41217547

DOI10.1021/acs.joc.2c02225

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.