Evidence map›Paper›PMID 41217301›Full record

ArticleACS synthetic biology2025

Disentangling Metabolite Detection from Pathway Stress Using Biosensors.

Gregory Donovan, Nolan O'Connor, Amanda M Moravek, Maggie Fox, Andrew L Markley, Tom Foderaro, Joel M Kralj, Jerome M Fox

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Gregory DonovanDepartment of Chemical and Biological Engineering, University of Colorado, Boulder, 3415 Colorado Avenue, Boulder, Colorado 80303, United States.
Nolan O'ConnorThink Bioscience, 1745 38th Street, Ste 150, Boulder, Colorado 80301, United States.
Amanda M MoravekThink Bioscience, 1745 38th Street, Ste 150, Boulder, Colorado 80301, United States.
Maggie FoxDepartment of Chemical and Biological Engineering, University of Colorado, Boulder, 3415 Colorado Avenue, Boulder, Colorado 80303, United States.
Andrew L MarkleyThink Bioscience, 1745 38th Street, Ste 150, Boulder, Colorado 80301, United States.
Tom FoderaroThink Bioscience, 1745 38th Street, Ste 150, Boulder, Colorado 80301, United States.
Joel M KraljThink Bioscience, 1745 38th Street, Ste 150, Boulder, Colorado 80301, United States.
Jerome M FoxDepartment of Chemical and Biological Engineering, University of Colorado, Boulder, 3415 Colorado Avenue, Boulder, Colorado 80303, United States.ORCID 0000-0002-3739-1899

Funding

Microbially guided discovery and biosynthesis of biologically active natural productsR35GM143089 · NIGMS · UNIVERSITY OF COLORADO · PI FOX, JEROME · 2021 to 2025
$1.8M
National Institute of General Medical Sciences of the National Institutes of HealthNIGMS NIH HHS R35 GM143089
6 · The paper itself

Abstract

Genetically encoded biosensors are powerful tools for linking metabolite production to fluorescent outputs, but stresses imposed by engineered pathways can confound their signals. In this study, we developed four fluorescence-based biosensors for inhibitors of protein tyrosine phosphatase 1B (PTP1B), a therapeutic target for cancer and diabetes, and compared their performance in the presence of heterologous terpenoid pathways, a promising source of new inhibitors. When expressed in

Indexed as

Biosensing TechniquesProtein Tyrosine Phosphatase, Non-Receptor Type 1DNA-Directed RNA PolymerasesEscherichia coliHumansStress, PhysiologicalTerpenesViral Proteinsbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesProtein Tyrosine Phosphatase, Non-Receptor Type 1PTPN1 protein, humanTerpenesViral Proteinsbacterial two-hybridbiosynthetic pathwayscellular burdenenzyme inhibitorsprotein tyrosine phosphatasesT7 RNA polymerase

Identifiers

PMID41217301
PMCPMC13271753

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.