ArticleVeterinary medicine (Auckland, N.Z.)2025
Development of a Novel Loop-Mediated Isothermal Amplification (LAMP) for Rapid Diagnosis of Newcastle Disease in Field and Resource-Limited Areas.
Article in Veterinary medicine (Auckland, N.Z.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Newcastle Disease (ND) is a highly contagious and fatal poultry disease caused by Newcastle disease virus (NDV) and has a global distribution. To control this disease, a rapid method for diagnosing it is needed. PCR-based molecular diagnostics such as real-time reverse transcription-PCR (RRT-PCR) for detecting NDV genes have been conducted worldwide. However, because PCR methods are time-consuming and require a well-equipped laboratory, they are not well-suited for use in field and resource limited areas. Methods: In this study, we established a field-friendly loop-mediated isothermal amplification (LAMP) assay for NDV diagnosis using primers targeting the highly conserved L gene, which bypasses the need for RNA extraction and makes it more suitable for field application than previously reported LAMP methods. Results: Our LAMP method is capable of detecting a broad range of NDV genotypes and showed no cross-reactivity with other avian viral diseases or the host genome. The reaction is completed within 35 minutes of incubation at 65°C. NDV was successfully detected directly from swab and tissue samples without the need for conventional RNA extraction. Conclusion: The LAMP method developed in this study offers a rapid, affordable, and field-friendly diagnostic tool for NDV detection.
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