Evidence map›Paper›PMID 41213346›Full record

ArticleFish & shellfish immunology2026

Use of shotgun immunoproteomics for the development of protein vaccines against Edwardsiella piscicida.

Kim L Jacobsen, Matt J Griffin, Brett S Phinney, Michelle Salemi, Zeinab Yazdi, Sujita Balami, Caitlin E Older, Cynthia C Ware, Fernando Y Yamamoto, Noor-Ul Huda and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fish & shellfish immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Kim L JacobsenDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Matt J GriffinThad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, 38776, USA.
Brett S PhinneyProteomics Core Facility, University of California, Davis, CA, 95616, USA.
Michelle SalemiProteomics Core Facility, University of California, Davis, CA, 95616, USA.
Zeinab YazdiDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA.
Sujita BalamiThad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, 38776, USA.
Caitlin E OlderWarmwater Aquaculture Research Unit, Agricultural Research Service, U.S. Department of Aquaculture, Stoneville, MS, 38776, USA.
Cynthia C WareThad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, 38776, USA.
Fernando Y YamamotoThad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, 38776, USA; Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, Mississippi State, MS, USA.
Noor-Ul HudaThad Cochran National Warmwater Aquaculture Center, Mississippi State University, Stoneville, MS, 38776, USA.
Tania Rodriguez-RamosDepartment of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Brian DixonDepartment of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Jie MaAquatic Animal Health Research Unit, USDA-ARS, Auburn, AL, 36832, USA.
Benjamin R LaFrentzAquatic Animal Health Research Unit, USDA-ARS, Auburn, AL, 36832, USA.
Esteban SotoDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA. Electronic address: sotomartinez@ucdavis.edu.

Funding

Comparative Medical Science Training ProgramT32OD011147 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI JEROEN SAEIJ, Sara Michelle Thomasy · 2012 to 2026
$5.5M
QE-HF-X Mass SpectrometerS10OD026918 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2020 to 2020
$600k
NIH HHS S10 OD026918NIH HHS T32 OD011147
6 · The paper itself

Abstract

Edwardsiella piscicida is an important emerging pathogen in various cultured fish species. This study aimed to identify immunogenic E. piscicida proteins and evaluate these antigens as protein vaccines for use in aquaculture. Shotgun immunoproteomics using anti-E. piscicida serum from rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus) (♀) × blue catfish (Ictalurus furcatus) (♂) hybrids inoculated with formalin-killed whole-bacteria preparations identified 36 candidate immunogenic E. piscicida proteins. The chaparonin GroEL, the glycine 2TM zipper domain-containing protein (GlyZip), and coproporphyrinogen-III oxidase (COPIII) were used to orally (PO) and intra-coelomically (IC) immunize Chinook salmon (Oncorhynchus tshawytscha). Fish IC vaccinated with either GlyZip or COPIII demonstrated a slight, but non-significant, improvement in survival post-challenge with E. piscicida S11-285. Surprisingly, fish IC or PO vaccinated with GroEL displayed an anti-protective effect (RPS = -184 % and RPS = -76 %, respectively) against subsequent challenge. All IC vaccinated fish generated a strong specific antibody response against the immunizing protein, and sham vaccinated fish challenged with E. piscicida S11-285 generated a significantly higher specific antibody response to the GroEL and GlyZip proteins than negative control fish, suggesting that shotgun immunoproteomics was effective for detection of immunogenic bacterial proteins that can stimulate humoral immune responses in the host fish.

Indexed as

Bacterial ProteinsBacterial VaccinesEdwardsiellaEnterobacteriaceae InfectionsFish DiseasesIctaluridaeOncorhynchus mykissAnimalsProteomicsBacterial ProteinsBacterial VaccinesAquacultureCatfishEdwardsiellosisRecombinant vaccineSalmonidsSubunit vaccine

Identifiers

PMID41213346
PMCPMC13542899

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.