Evidence map›Paper›PMID 41213020›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Genome synthesis, assembly, and rebooting of therapeutically useful high G+C% mycobacteriophages.

Ching-Chung Ko, Andrew P Sikkema, Michael J Lauer, Elizabeth D Amarh, Rebecca A Garlena, Daniel A Russell, Nicole Chew, Tan Li, Isa Madrigal Harrison, Jared Ellefson and 2 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Realizing phage therapy in the UK.Nature microbiology · 2026
    Article
  6. A fully synthetic Golden Gate assembly system for engineering aProceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ching-Chung KoDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
Andrew P SikkemaResearch Department, Applied Molecular Biology, New England Biolabs, Ipswich, MA 01938.
Michael J LauerDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
Elizabeth D AmarhDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
Rebecca A GarlenaDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
Daniel A RussellDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.
Nicole ChewAnsa Biotechnologies, Emeryville, CA 94608.
Tan LiAnsa Biotechnologies, Emeryville, CA 94608.
Isa Madrigal HarrisonAnsa Biotechnologies, Emeryville, CA 94608.
Jared EllefsonAnsa Biotechnologies, Emeryville, CA 94608.
Graham F HatfullDepartment of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260.ORCID 0000-0002-6705-6821
Gregory J S LohmanResearch Department, Applied Molecular Biology, New England Biolabs, Ipswich, MA 01938.

Funding

Bacteriophage diversity, dynamics, function, and exploitationR35GM131729 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Graham F. Hatfull · 2019 to 2026
$3.6M
Dynamics of viral host range evolutionR01GM116884 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HATFULL, GRAHAM F. · 2015 to 2019
$2.0M
HHS | NIH (NIH) GM116884Howard Hughes Medica GT12053NIGMS NIH HHS R01 GM116884NIGMS NIH HHS R35 GM131729
6 · The paper itself

Abstract

Bacteriophages show therapeutic promise for treating bacterial pathogens including nontuberculous mycobacteria (NTM). A major impediment is the paucity of therapeutically useful phages and the great variation in the phage infection profiles, especially among

Indexed as

DNA, ViralGenome, ViralMycobacteriophagesBase CompositionMycobacterium abscessusMycobacterium smegmatisDNA, Viralbacteriophagesphage therapysynthetic genomics

Identifiers

PMID41213020
PMCPMC12646305

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.