ArticleThe Journal of organic chemistry2025
Coumarin-Based Photolabile Solid Support Facilitates Nonchromatographic Purification of RNA Oligonucleotides.
Article in The Journal of organic chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Improved Purification of sgRNA and pegRNA by Use of a 5'-Terminal Dioctyloxytrityl (DOT) Protecting Group.The Journal of organic chemistry · 2026Article
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Authors and funding
4 authors.
Funding
Abstract
We describe the synthesis and application of a coumarin-based photocleavable anchor for solid-phase synthesis and nonchromatographic purification of RNA oligonucleotides. In contrast to standard nitroaryl anchors, which are cleaved with ultraviolet (UV) light, the described construct requires visible light (λ = 456 nm) for photocleavage. This is especially important for RNAs that contain a phosphorothioate backbone, which is not compatible with UV light. We optimized the synthesis and purification process using a model 20-nt poly-U RNA and subsequently applied it to structured and unstructured RNA oligonucleotides that contain both phosphodiester and phosphorothioate backbones. Lastly, we purified 103-nt sgRNA and checked its functional fidelity by CRISPR experiments.
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