A modified hot phenol-based protocol for high-purity Escherichia coli lipopolysaccharide extraction: Biochemical validation, stem cell cytotoxicity, and dose dependent multi-organ inflammation in a rat model.

Edris Vahdani, Ali Sepehrinezhad, Elham Hosseini, Saman Soleimanpour, Sajad Sahab Negah, Mohammad Ahanjan

Abstract read

In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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0 citing papers in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Edris VahdaniDepartment of Medical Microbiology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Ali SepehrinezhadNeuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Elham HosseiniNeuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Saman SoleimanpourDepartment of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Iran.

Sajad Sahab NegahNeuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Mohammad AhanjanImmunogenic Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gram-negative bacteria contain lipopolysaccharides (LPS) in their outer membrane, which induce strong inflammatory responses. Traditional LPS extraction methods often leave residual protein and nucleic acid contamination. These impurities interfere with downstream analyses and reduce reproducibility in biological studies. This study provides a modified hot phenol method combined with enzymatic treatments using proteinase K, DNase, RNase to extract pure LPS from

Indexed as

E. coliEmbryonic neural stem cellInflammationLipopolysaccharideModified hot phenol methodOrgan damageRats

Identifiers

PMID41210196

DOI10.1016/j.btre.2025.e00933

PMCPMC12589890

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.