ArticleEcology and evolution2025
Establishing DNA-Based Strategies for Soil Biodiversity Assessment: Insights From Carabid Beetles.
Article in Ecology and evolution, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Establishing DNA-Based Strategies for Soil Biodiversity Assessment: Insights From Carabid Beetles.Ecology and evolution · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular-based methods offer valuable opportunities for assessing soil biodiversity in different ecosystems. However, their reliability and large-scale applicability depend on developing, optimizing protocols and establishing high quality, curated local reference databases. This study aimed to evaluate key steps in soil macroinvertebrate metabarcoding workflow, including the sample decontamination process and the efficiency of taxa recovery. Specifically, we sought to: (1) determine the impact of sample decontamination, (2) validate species-level recovery efficiency of the metabarcoding pipeline spiked with a curated mock community of morphologically identified and barcoded carabid beetles, and (3) compare traditional morphological identification and metabarcoding for specimens' taxonomic assignment and recovery. Our results showed that the commonly used decontamination process did not significantly impact OTU richness, suggesting it is not essential for this fauna. Compared to morphology, to metabarcoding provided a more comprehensive taxonomic overview at higher level taxa. However, validation with the mock community revealed discrepancies in species-level recovery, underscoring that its accuracy is highly contingent on the quality of the reference database. DNA metabarcoding is a currently used and promising technique for macroinvertebrate assessment regarding time, efficiency, and costs, yet reaching greater depth in taxonomic resolution. Yet, its species-level accuracy remains dependent on comprehensive and well-curated barcode reference databases. We recommend an integrative approach, combining molecular data with targeted validation, for the most robust outcomes. For this reason, we recommend the use of integrative methodologies for robust and rapid biodiversity assessments. We found that the common decontamination step is not crucial for soil macrofauna metabarcoding accuracy. Consequently, its removal streamlines sample processing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.