Evidence map›Paper›PMID 41206868›Full record

ArticleSTAR protocols2025

Protocol for the quantification of mouse fecal IgM, IgG, and IgA utilizing standard curve-based enzyme-linked immunosorbent assays.

KimAnh T Pioli, Peter D Pioli

Abstract read
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

KimAnh T PioliDepartment of Biochemistry, Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada. Electronic address: kim.pioli@usask.ca.
Peter D PioliDepartment of Biochemistry, Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada. Electronic address: peter.pioli@usask.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunoglobulin (Ig) transport into the intestinal lumen is of interest as Igs modulate gut microbial species composition and play protective roles upon exposure to intestinal pathogens. Here, we present a protocol for the quantification of mouse intestinal Igs through enzyme-linked immunosorbent assay (ELISA) measurement. We describe procedures for isolating and processing feces from the whole mouse intestine. We then detail steps for the quantification of mouse fecal IgM, IgG, and IgA using the generated ELISA standard curves. For complete details on the use and execution of this protocol, please refer to Pioli et al.

Indexed as

FecesImmunoglobulin AImmunoglobulin GImmunoglobulin MAnimalsEnzyme-Linked Immunosorbent AssayMiceImmunoglobulin AImmunoglobulin GImmunoglobulin MAntibodyHealth SciencesImmunologyModel OrganismsMolecular Biology

Identifiers

PMID41206868
PMCPMC12648601

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.