ArticleCurrent microbiology2025
Development and Application of a Species-Specific PCR Assay for the Detection of the Emerging Food-Borne Pathogen Cronobacter Sakazakii.
Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Cronobacter sakazakii is an emerging opportunistic food-borne pathogen associated with a wide range of foods including seafood, meat, plant-based, and dairy products. Although seafood is a significant source of this pathogen, specific and sensitive methods that can differentiate C. sakazakii from other Cronobacter species are currently not available. In this study, a PCR assay using primers targeting fhuF gene was developed and evaluated for its specificity and sensitivity in detecting C. sakazakii. The designed primers exhibited high level of specificity for C. sakazakii and no cross reaction was observed with other Cronobacter species and related Enterobacterales. The sensitivity of detection was tested using pure culture of C. sakazakii and in shrimp samples artificially spiked with C. sakazakii. The fhuF-specific PCR assay could detect as low as 1.45 × 10
Indexed as
Identifiers
41204960What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.