Evidence map›Paper›PMID 41204349›Full record

ArticleJournal of translational medicine2025

Multi-omics analyses identify mannose phosphate isomerase-centered hypoxia-induced angiogenesis signature in colorectal cancer.

Sicheng Liu, Yang Zhang, Yang Meng, Qing Huang, Zijun Feng, Linda Wen, Xuyang Yang, Yaguang Zhang, Lei Qiu, Ziqiang Wang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sicheng Liu *Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Yang Zhang *Colorectal Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Yang MengDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Qing HuangDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Zijun FengDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Linda WenDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Xuyang YangColorectal Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Yaguang ZhangDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Lei QiuDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Ziqiang WangColorectal Cancer Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
Bo ZhangLaboratory of Gastrointestinal Tumor Epigenetics and Genomics, Department of General Surgery, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Zhixin ChenLaboratory of Gastrointestinal Tumor Epigenetics and Genomics, Department of General Surgery, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China.
Junhong HanDepartment of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu, 610041, China. hjunhong@scu.edu.cn.ORCID 0000-0002-3371-8698

Funding

1·3·5 Project for Disciplines of Excellence, West China Hospital ZYGD23017Fellowship of China National Postdoctoral Program for Innovative Talents, China Postdoctoral Science Foundation BX20230244Foundation for Innovative Research Groups of the National Natural Science Foundation of China 81820012Natural Science Foundation of Sichuan Province 2023NSFSC0719Sichuan Science and Technology Program MZGC20240001
6 · The paper itself

Abstract

backgroundHypoxia and angiogenesis are crucial hallmarks of cancer that play key roles in the development and progression of colorectal cancer (CRC). However, the transcriptional mechanism underlying hypoxia induced angiogenesis (HIA) remain elusive. This study aimed to explore the regulatory networks, molecular mechanisms, and prognostic value of HIA-related genes.

methodsWe collected multi-omics data, including chromatin immunoprecipitation sequencing (ChIP-seq), bulk RNA-seq, single cell RNA-seq, spatial transcriptomics, and microarray data from CRC patients and cell lines. Computational methods, including single sample gene set enrichment analysis (ssGSEA), signature-related gene analysis (SRGA), consensus clustering, and others, were utilized to explore the correlation between hypoxia and angiogenesis, identify the HIA-related genes, and establish the risk scoring system based on HIA-related genes. The role of mannose phosphate isomerase (MPI) was validated using quantitative real-time PCR (RT-qPCR), Co-immunoprecipitation (Co-IP), western blot, colony formation, tube formation assay, and subcutaneous xenograft tumor models in vitro and in vivo.

resultsWe identified 12 HIA-related genes that are transcriptionally activated by hypoxia-inducible factors (HIFs) and functionally implicated in angiogenesis in CRC. Based on the differentially expressed genes among HIA-related CRC subtypes, we constructed a prognostic scoring system termed HIAscore. Patients with high HIAscore was correlated with poor survival, aggressive phenotype, and immunosuppressive tumor microenvironment. Spatial analysis revealed sequestration regions between epithelial cells with higher HIAscore and T/I/NK cells, hindering their infiltration. Particularly, MPI was found to interact with lactate dehydrogenase A (LDHA), and promote proliferation and angiogenesis of CRC through phosphorylation and activation of Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) signaling pathways.

conclusionsThis study depicts the transcriptional landscape linking hypoxia and angiogenesis in CRC, and identifies MPI as a novel regulator of this process.

Indexed as

Colorectal NeoplasmsHypoxiaNeovascularization, PathologicAngiogenesisAnimalsCell HypoxiaCell Line, TumorGene Expression ProfilingGene Expression Regulation, NeoplasticGene Regulatory NetworksHumansMiceMultiomicsReproducibility of ResultsColorectal cancerHypoxia and angiogenesisMannose phosphate isomerase (MPI)Prognostic signatureRegulatory network

Identifiers

PMID41204349
PMCPMC12595641

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.