Evidence map›Paper›PMID 41204281›Full record

ArticleStem cell research & therapy2025

Inhibition of Galectin-3 by ADSC-derived exosomes after cavernous nerve injury ameliorates erectile dysfunction by modulating oxidative stress and inflammatory profibrotic cascades.

Ruiyu Li, Guanbo Wang, Chen Feng, Tongxiang Diao, Shuai Liu, Qiang Fu

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Ruiyu Li *Department of Urology, Shandong Provincial Hospital, Shandong University, Jinan, 250021, Shandong, China.
Guanbo Wang *Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.
Chen FengDepartment of Urology, Shandong Provincial Hospital, Shandong University, Jinan, 250021, Shandong, China.
Tongxiang DiaoDepartment of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.
Shuai LiuDepartment of Urology, Shandong Provincial Hospital, Shandong University, Jinan, 250021, Shandong, China. liushuai@sdfmu.edu.cn.
Qiang FuDepartment of Urology, Shandong Provincial Hospital, Shandong University, Jinan, 250021, Shandong, China. qiangfu68@163.com.ORCID http://orcid.org/0000-0002-1834-0041

Funding

Jinan Science and Technology Plan 202134010National Natural Science Foundation of China 82071635Natural Science Foundation of Shandong Province ZR2021QH366Natural Science Foundation of Shandong Province ZR2023MH019Shandong Province Medical and Health Development Plan 202004051394
6 · The paper itself

Abstract

backgroundCavernous nerve injury (CNI) is a prominent etiological factor in the development of erectile dysfunction (ED). Nevertheless, the underlying pathophysiological mechanisms of CNI-induced ED (CNI-ED) are not fully elucidated. Galectin-3 (Gal-3), an indicator of inflammation and fibrosis, has been implicated in the pathogenesis of arteriogenic ED. In this study, we investigate the role of Gal-3 in CNI-ED and explore the potential mechanisms by which adipose-derived mesenchymal stem cell exosomes (ADSC-Exo) ameliorate CNI-ED.

methodsWe established a bilateral CNI (BCNI) rat model and evaluated erectile function using intracavernous pressure (ICP), mean arterial pressure (MAP), and infrared ray thermography (IRT). The expression level of Gal-3 was measured in the major pelvic ganglia (MPG), penile corpus cavernosum, and cultured cells, with its expression modulated by lentiviral vectors. A combination of experimental approaches, including western blot, immunofluorescence, and flow cytometry analysis, were employed to investigate the role of Gal-3 in the progression of CNI-ED. Additionally, the potential molecular mechanisms by which ADSC-Exo ameliorates CNI-ED were explored.

resultsOur findings indicated that the expression of Gal-3 is significantly upregulated in the MPG and penile corpus cavernosum of BCNI rats. This upregulation was accompanied by oxidative stress and activation of the TLR4/MyD88/NF-κB signaling pathway. Following lentiviral knockdown of Gal-3, erectile function in BCNI rats was improved. Moreover, ADSC-Exo transplantation inhibited Gal-3 expression and the related inflammatory profibrotic cascades.

conclusionsCollectively, this study demonstrates that upregulation of Gal-3 promotes the pathogenesis of CNI-ED by triggering oxidative stress and inflammatory profibrotic cascades and highlights the therapeutic potential of ADSC-Exo in restoring erectile function via Gal-3 inhibition.

Indexed as

Erectile DysfunctionExosomesGalectin 3Mesenchymal Stem CellsPeripheral Nerve InjuriesAnimalsFibrosisInflammationMaleNF-kappa BOxidative StressPenisRatsRats, Sprague-DawleySignal TransductionToll-Like Receptor 4Galectin 3Lgals3 protein, ratNF-kappa BToll-Like Receptor 4Adipose mesenchymal stem cell-derived exosomesErectile dysfunctionGalectin-3Inflammatory profibrotic cascadesOxidative stress

Identifiers

PMID41204281
PMCPMC12595703

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