Evidence map›Paper›PMID 41204088›Full record

ArticleBMC plant biology2025

SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning.

Anthony S Wokasch, Owen Albanese, Joshua Terao, Maryam Yamadi, Aidan Deneen, Ashley Bastin, Sofia I Palau, Jose Calvo Palacios, Charlotte S Foege, Mariam R Coulibaly and 1 more

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In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Anthony S WokaschDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Owen AlbaneseDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Joshua TeraoDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Maryam YamadiDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Aidan DeneenDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Ashley BastinDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Sofia I PalauDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Jose Calvo PalaciosDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Charlotte S FoegeDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Mariam R CoulibalyDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA.
Naden T KroganDepartment of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC, 20016, USA. nkrogan@american.edu.ORCID http://orcid.org/0000-0002-9104-2665

Funding

Mechanisms Controlling Cellular Differentiation and Proliferation in a Plant Stem Cell MicroenvironmentR15GM114733 · NIGMS · AMERICAN UNIVERSITY · PI KROGAN, NADEN · 2015 to 2019
$742k
Division of Integrative Organismal Systems 1916804NIGMS NIH HHS 2R15GM114733-02NIGMS NIH HHS R15 GM114733
6 · The paper itself

Abstract

backgroundThe spatial regulation of gene expression is crucial for the development of complex structures such as the angiosperm flower. The combinatorial actions of floral organ identity genes, which are expressed in well-defined domains, specify the fate of lateral organs arranged in concentric whorls in the flower. SUPERMAN (SUP) contributes to this regulation by establishing a boundary between floral whorls 3 and 4 in Arabidopsis, preventing both the central spread of APETALA3 and PISTILLATA gene expression and overproliferation of male reproductive organs. SUP operates as a transcriptional repressor and harbors an ERF-associated amphiphilic repression (EAR) motif.

resultsIn this work, we show that the corepressor TOPLESS (TPL) cooperates with SUP to repress APETALA3 and PISTILLATA and suppress male reproductive fate in the flower. Additionally, we demonstrate that SUP forms a physical interaction with TPL that is dependent on its EAR motif. We also specifically mutate the EAR motif of endogenous SUP in planta, showing that even a subtle change is sufficient to cause strong sup defects and interrupt TPL association.

conclusionsCollectively, our results indicate that SUP requires its EAR motif to recruit TPL and function as a cadastral regulator, with both proteins cooperatively establishing a gene expression boundary necessary for floral patterning.

Indexed as

ArabidopsisArabidopsis ProteinsFlowersTranscription FactorsAmino Acid MotifsGene Expression Regulation, PlantMADS Domain ProteinsAPETALA 3 protein, ArabidopsisArabidopsis ProteinsMADS Domain ProteinsPISTILLATA protein, Arabidopsissuperman protein, ArabidopsisTranscription FactorsArabidopsis thalianaEAR motifFloral organ identity genesFloral patterningGene regulationReproductive developmentSUPERMANTOPLESSTranscriptional repression

Identifiers

PMID41204088
PMCPMC12595690

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.