ArticleCommunications biology2025
The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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8 authors.
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Abstract
In eukaryotes, chromosome segregation relies on attachment to the spindle, ensured by the kinetochore. The outer kinetochore attaches to the microtubules and is named after three sub-complexes KNL1C, MIS12C, and NDC80C (KMN). While the KMN complex comprises ten proteins in humans S. cerevisiae, its conservation in more distant eukaryotes is unclear. Here, we aimed to define the KMN complex in the plant Arabidopsis using affinity purification and identified thirteen KMN proteins. Seven were previously known to have a conserved function (atMIS12, atNNF1, atNDC80, atSPC24, atSPC25, atNUF2, and atKNL1) and six were uncharacterized. These six proteins show remote similarity to yeast/human KMN-associated proteins, whose homologs have not yet been characterized in plants. We named them atDSN1, atCSM1, atNSL1.1/.2, and atZWINT1.1/.2. We confirmed kinetochore localization for atDSN1, atCSM1, atNSL1.1, and atZWINT1.1 in planta. In addition, atDSN1, atCSM1, and ZWINT1.1/.2 are essential, further supporting their kinetochore function. AlphaFold3 predicts an alike3D organization of the KMN complex in plants and mammals. We conclude that the KMN complex is globally conserved with a matching composition and similar organization in distant eukaryotes, with some local variations, suggesting its presence in the common ancestors of all living eukaryotes.
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