ArticleNature methods2025
nELISA: a high-throughput, high-plex platform enables quantitative profiling of the inflammatory secretome.
Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- dia-PASEF Enables Rapid Profiling of the Human Secretome for Deeper Insights Into Cellular Dynamics and Inflammatory Mechanisms.Molecular & cellular proteomics : MCP · 2026Article
- Serum proteomics in paediatric inflammatory bowel disease from a case-control study: biomarker discovery and ulcerative colitis-Crohn's disease differentiation.EBioMedicine · 2026Article
- Dissecting non-small cell lung cancer (NSCLC) with blood proteomics-from surgical to immunotherapeutic responses.NPJ precision oncology · 2026Article
- Transforming the cytokine literature into a resource for experimental analysis and discovery.bioRxiv : the preprint server for biology · 2026Article
- Progress and new challenges in image-based profiling.Molecular systems biology · 2026Review
- Ligand-Mediated Proteome Remodeling Shapes Nanoparticle Protein Corona Composition for Deep Plasma Profiling.Research square · 2026Article
- Temporal variation in markers of immune signaling and wound repair in primary human airway epithelial cells following acute versus repeated woodsmoke condensate exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Non-viral TcBuster transposon engineering of CD70-CAR natural killer cells for the treatment of osteosarcoma.Molecular therapy. Oncology · 2026Article
- WMRE2030: integrating wearable devices, multi-omics, and artificial intelligence-driven real-time feedback into a daily-scale closed-loop framework for a new era of precision exercise.Frontiers in physiology · 2026Review
- The OASIS Consortium: integrating multi-omics technologies to transform chemical safety assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
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- Morphological map of under- and overexpression of genes in human cells.Nature methods · 2025Article
- A morphology and secretome map of pyroptosis.Molecular biology of the cell · 2025Article
- A versatile information retrieval framework for evaluating profile strength and similarity.bioRxiv : the preprint server for biology · 2025Article
- Cell Painting: a decade of discovery and innovation in cellular imaging.Nature methods · 2025Review
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- AI discovery of TLR agonist-driven phenotypes reveals unique features of peripheral cells from healthy donors and ART-suppressed people living with HIV.Frontiers in immunology · 2025Article
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Abstract
Existing high-plex protein measurement tools compromise on quantification, precision and cost efficiency. Here, to address this, we present nELISA, a platform that combines a DNA-mediated, bead-based sandwich immunoassay with advanced multicolor bead barcoding. Antibody pairs are preassembled on target-specific, barcoded beads, which ensures spatial separation between noncognate assays. Detection antibodies are tethered via flexible single-stranded DNA to enable efficient ternary sandwich formation. Detection is achieved through toehold-mediated strand displacement, where fluorescently labeled DNA oligos simultaneously untether and label detection antibodies. nELISA delivers sub-picogram-per-milliliter sensitivity across seven orders of magnitude. Using a 191-plex inflammation panel, we profiled cytokine responses in 7,392 peripheral blood mononuclear cell samples, generating ~1.4 million protein measurements and revealing over 440 robust cytokine responses, including previously unreported effects. nELISA thus provides a simple, scalable and cost-efficient solution for large-scale, high-fidelity phenotypic screening.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.