Evidence map›Paper›PMID 41202818›Full record

ArticleStem cell reports2025

Dissecting Oct4 enhancer function in pluripotent stem cells and mouse embryogenesis.

Daniel A Schmitz, Daiji Okamura, Masahiro Sakurai, Yi Ding, Seiya Oura, Emily Ballard, Yulei Wei, Leqian Yu, Yingying Hu, Jun Wu

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Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Daniel A SchmitzDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Daiji OkamuraDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Advanced Bioscience, Faculty of Agriculture, Kindai University, Nara, Japan. Electronic address: dokamura@nara.kindai.ac.jp.
Masahiro SakuraiDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Yi DingDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Seiya OuraDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Emily BallardDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Yulei WeiDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Leqian YuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Yingying HuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Jun WuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA. Electronic address: jun2.wu@utsouthwestern.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

OCT4 is a master regulator of pluripotency, with expression restricted to pluripotent and germ cells. Its expression is controlled by two cis-regulatory elements: the distal (DE) and proximal (PE) enhancers. Although widely used as markers for pluripotent stem cells (PSCs), their biological roles have remained incompletely defined. Here, we generated PSC lines and mouse models with targeted deletions of the Oct4 DE and PE. Our findings reveal that the DE is dispensable for sustaining the primed pluripotent state but required for the naive state, whereas the PE is necessary for the primed state but not for the naive state. Notably, PE-deficient naive mouse PSCs retained the capacity to differentiate into somatic lineages in vitro and to contribute chimeras. In contrast, deletion of either enhancer in vivo resulted in early embryonic lethality. These models offer powerful genetic tools to dissect the regulation of Oct4 expression during pluripotency and early development.

Indexed as

Embryonic DevelopmentEnhancer Elements, GeneticOctamer Transcription Factor-3Pluripotent Stem CellsAnimalsCell DifferentiationGene Expression Regulation, DevelopmentalMiceOctamer Transcription Factor-3Pou5f1 protein, mousechimerasenhancerformative pluripotencynaive pluripotencyOCT4pluripotent stem cellsPOU5F1primed pluripotency

Identifiers

PMID41202818
PMCPMC12744841

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