Evidence map›Paper›PMID 41201087›Full record

ArticleCell reports2025

Poly-ubiquitylated transmembrane proteins outcompete other cargo for limited space inside clathrin-coated vesicles.

Hao-Yang Liu, Susovan Sarkar, Feng Yuan, Grant Ashby, Carl C Hayden, Jon M Huibregtse, Jeanne C Stachowiak

Abstract read
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In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Hao-Yang LiuDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Susovan SarkarDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Feng YuanDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Grant AshbyDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Carl C HaydenDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Jon M HuibregtseDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
Jeanne C StachowiakDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. Electronic address: jcstach@austin.utexas.edu.

Funding

Mechanism and Function of ISG15 ConjugationR01AI096090 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI HUIBREGTSE, JON · 2011 to 2025
$5.9M
Protein Networks as Synergistic Drivers of Membrane RemodelingR35GM139531 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Jeanne Casstevens Stachowiak · 2021 to 2026
$4.0M
NIAID NIH HHS R01 AI096090NIGMS NIH HHS R35 GM139531
6 · The paper itself

Abstract

Endocytic recycling of transmembrane proteins is essential to cellular function. The intracellular domains of transmembrane proteins are frequently ubiquitylated, a modification that is recognized by adaptor proteins during clathrin-mediated endocytosis. Recent work suggests that transmembrane proteins compete for space within highly crowded endocytic structures, suggesting that enhanced internalization of one group of transmembrane proteins may come at the expense of others. Here, we show that preferential internalization of poly-ubiquitylated transmembrane proteins can result in reduced endocytosis of mono-ubiquitylated and non-ubiquitylated proteins. Further, poly-ubiquitylated receptors significantly outcompeted their less ubiquitylated counterparts for uptake of extracellular ligands. These findings suggest that clathrin-coated vesicles may act as selective filters, prioritizing highly ubiquitylated transmembrane proteins for uptake while leaving others behind. Given that poly-ubiquitylation is thought to signal protein aging and damage, these findings suggest a mechanism for selective internalization of high priority cargo, with simultaneously exclusion and protection of functional proteins lacking poly-ubiquitylation.

Indexed as

Clathrin-Coated VesiclesMembrane ProteinsUbiquitinationAnimalsEndocytosisHEK293 CellsHeLa CellsHumansMembrane ProteinsclathrinCP: Cell biologyendocytosismembrane traffictransmembrane proteinubiquitylation

Identifiers

PMID41201087
PMCPMC12727139

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.