Evidence map›Paper›PMID 41201025›Full record

ArticleInternational journal of molecular medicine2026

ROCK inhibition promotes axon and myelin regeneration via PI3K/Akt/GSK3β in a mouse sciatic nerve injury model.

Shuang Dou, Zhijun Li, Boyao Zheng, Zhenyu Ren, Hai Wang, Qing Zuo, Fang Fang, Yuehong Zhuang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuang Dou *Institute of clinical applied anatomy, Fujian Key Laboratory of brain aging and neurodegenerative diseases, School of basic medical sciences, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.
Zhijun Li *Department of pharmacology, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.
Boyao ZhengInstitute of clinical applied anatomy, Fujian Key Laboratory of brain aging and neurodegenerative diseases, School of basic medical sciences, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.
Zhenyu RenOrthopedic Department, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350108, P.R. China.
Hai WangOrthopedic Department, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian 350108, P.R. China.
Qing ZuoInstitute of clinical applied anatomy, Fujian Key Laboratory of brain aging and neurodegenerative diseases, School of basic medical sciences, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.
Fang FangDepartment of pharmacology, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.
Yuehong ZhuangInstitute of clinical applied anatomy, Fujian Key Laboratory of brain aging and neurodegenerative diseases, School of basic medical sciences, Fujian medical university, Fuzhou, Fujian 350122, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study investigates the molecular mechanisms of peripheral nerve regeneration by examining the ROCK/PI3K/Akt/GSK3β pathway's role in promoting morphological and functional recovery after peripheral nerve injury (PNI). Using a mouse sciatic nerve crush (SNC) injury model and a dorsal root ganglion (DRG) explant axotomy model, mice and DRG were divided the experimental (treated with DMSO) group, Y27632 group (treated with ROCK inhibitor Y27632), Y + LY group (treated with Y27632 + PI3K inhibitor LY294002), and Y + LY + SB group (treated with Y27632 + LY294002 + GSK3β inhibitor SB216763). Immunofluorescence was used to assess axon density, diameter, myelin thickness and Schwann cell proliferation, while retrograde tracing with cholera toxin subunit B evaluated peripheral‑to‑central reconnection. Behavioral tests measured functional recovery, and in DRG explants, axon regeneration length and growth cone size were quantified. Protein expression analysis of RhoA, ROCK, PI3K, Akt, GSK3β, and their phosphorylated forms was conducted on day 3 post‑axotomy, both

Indexed as

AxonsGlycogen Synthase Kinase 3 betaMyelin SheathNerve RegenerationPeripheral Nerve InjuriesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktrho-Associated KinasesSciatic NerveAmidesAnimalsDisease Models, AnimalGanglia, SpinalMaleMiceMice, Inbred C57BLAmidesGlycogen Synthase Kinase 3 betaMorpholinesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktPyridinesrho-Associated KinasesY 27632axon regenerationgrowth coneperipheral nerve injuryremyelinationROCK/PI3K/Akt/GSK3β

Identifiers

PMID41201025
PMCPMC12594516

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.