ArticleJournal of cell science2025
Ubiquitylation-dependent Rap2 activation regulates lamellipodia dynamics during cell migration.
Article in Journal of cell science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Non-canonical and constitutive activation of small GTPases: more than an exception to the rule?The Biochemical journal · 2026Review
- Rap1 activity and localization is regulated by Rab40/CRL5 facilitated mono-ubiquitylation.microPublication biology · 2025Article
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Authors and funding
7 authors.
Funding
Abstract
Cell migration is a complex process hallmarked by front-to-back cell polarity that is established by the highly dynamic actin cytoskeleton. Branched actin polymerization creates a lamellipodium at the leading edge of the cell, while the contractile acto-myosin cytoskeleton is present at the lagging edge. Rap2, a Ras GTPase family member, has previously been reported to localize to the lamellipodium as a result of ubiquitylation by a Rab40-Cullin5 E3 ubiquitin ligase complex (Rab40/CRL5). However, how Rap2 functions and how ubiquitylation targets Rap2 to the lamellipodium remained unclear. Here, we demonstrate that Rap2 is recruited to retracting lamellipodia ruffles where it inhibits RhoA, likely through interactions with ARHGAP29, and regulates lamellipodia dynamics, thus facilitating cell migration. Furthermore, using a variety of genetic and pharmacological techniques, we show that Rab40/CRL5-dependent ubiquitylation is required for guanine-nucleotide-exchange factor (GEF)-dependent Rap2 activation, a necessary step for Rap2 targeting to the lamellipodium membrane. As such, we demonstrate how this unique ubiquitylation and activation of Rap2 regulates lamellipodia actin dynamics during cell migration.
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