ArticleOpen veterinary journal2025
Morpho-molecular characterization of Trypanosoma evansi in sheep.
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Aim: This study was established to evaluate the prevalence of Methods: A total of 200 blood samples were obtained from October 2023 to March 2024. These samples were examined using Giemsa stain under a light microscope, and 40 positive samples were selected for further investigation using conventional polymerase chain reaction (PCR). Results: The results showed an infection rate of 20%, with significant differences observed between male and female sheep. Younger sheep were found to be significantly more affected than older ones. Ten PCR-detected samples were randomly selected for DNA molecular analysis to obtain ITS-1 gene nucleotide sequences. The PCR product exhibited a band size of 1,264 bp, and the sequences were deposited in the National Center for Biotechnology Information under the following code numbers: PP930358.1, PP930353.1, PP930350.1, PP930357.1, PP930356.1, PP930351.1, PP930359.1, PP930352.1, PP930354.1, and PP930355.1. Conclusion: The current study results indicate that
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