ArticleOpen veterinary journal2025
Analysis of porcine gelatin in gelatin powder and gelatin-containing foods using non-targeted LC-Orbitrap HRMS-based proteomics.
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Photocrosslinkable lung dECM hydrogels promote stiffness-dependent lung cancer growth and chemoresistance.Materials today. Bio · 2026Article
- Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment.Frontiers in pharmacology · 2026Article
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Authors and funding
11 authors.
Funding
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Abstract
Background: Detecting porcine gelatin in gelatin powders and food products is crucial for ensuring compliance with dietary restrictions, ethical considerations, and regulatory standards. Aim: This study presents a non-targeted proteomic approach utilizing liquid chromatography coupled with high-resolution Orbitrap mass spectrometry to identify and quantify porcine gelatin in gelatin powder and food products, such as gummy candies and marshmallows. Methods: Peptide sequences unique to bovine and porcine gelatin were identified through comparative mass spectrometry data analysis, enabling reliable differentiation from gelatin sourced from other animals. Both extraction buffers, including Tris-HCl buffer (pH 8.0) and ammonium bicarbonate (pH 8.0), were effective for protein and peptide extraction in gelatin. Several porcine gelatin peptide markers were obtained in both extraction buffers. In addition, this study revealed that the reduction and alkylation processes using dithiothreitol and iodoacetamide, respectively, are unnecessary, thereby simplifying the extraction steps. Results: This method was successfully tested with gelatin used in complex food matrices such as gummy candies and marshmallows. Porcine gelatin was detected in three samples of gummy candies and three samples of marshmallows. Conclusion: Therefore, future research, such as the targeted approach and standardization, is strongly required to obtain a valid, reliable, and reproducible method for the analysis of porcine gelatin in food and pharmaceutical product applications for halal authentication purposes.
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