Evidence map›Paper›PMID 41200136›Full record

ArticleBiology methods & protocols2025

Enhanced protocol for measuring glutathione peroxidase activity using a new glutathione peroxidase-Tiron assay.

Mahmoud Hussein Hadwan, Abbas Ali Mohammed, Saeed Najavand, Roaa Altaee, Asad M Hadwan

Abstract read
In one paragraph

Article in Biology methods & protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mahmoud Hussein HadwanDepartment of Chemistry, College of Science, University of Babylon, Hilla City 51001, Iraq.ORCID https://orcid.org/0000-0003-1958-7764
Abbas Ali MohammedDepartment of Chemistry, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran.
Saeed NajavandDepartment of Chemistry, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran.
Roaa AltaeeCollege of Pharmacy, Al-Zahraa University for Women, Karbala Governorate 11118, Iraq.
Asad M HadwanCollege of Dentistry, University of Manara, Al-Amarah 12218, Iraq.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional methods for measuring glutathione peroxidase (GPx) activity are limited by interference issues, complex protein precipitation steps, and variable reliability, necessitating the development of improved analytical approaches for both research and clinical applications. A modified GPx activity assay has been developed utilizing the Tiron reagent system, which eliminates the need for protein precipitation. The protocol employs a novel termination reagent containing ferrous ion (Fe

Indexed as

clinical diagnosticsenzyme activity assayglutathione peroxidaseoxidative stressspectrophotometrytiron reagent

Identifiers

PMID41200136
PMCPMC12587416

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.