Evidence map›Paper›PMID 41196494›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Visualization and Quantification of Basal Melanocytes Within a 3D Bioengineered Skin Equivalent.

Nicole Barrett, Mia Lindsay, Paola De Los Santos Gomez, Jessica Simpson, Victoria Maltman, Kirsty Goncalves, Stefan Przyborski

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nicole BarrettDepartment of Biosciences, Durham University, Durham, UK.
Mia LindsayDepartment of Biosciences, Durham University, Durham, UK.
Paola De Los Santos GomezDepartment of Biosciences, Durham University, Durham, UK.
Jessica SimpsonDepartment of Biosciences, Durham University, Durham, UK.
Victoria MaltmanDepartment of Biosciences, Durham University, Durham, UK.
Kirsty GoncalvesDepartment of Biosciences, Durham University, Durham, UK.
Stefan PrzyborskiDepartment of Biosciences, Durham University, Durham, UK. stefan.przyborski@durham.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Determining the melanogenic capacity of basal melanocytes is vital when studying skin pigmentation and related disorders. Human skin equivalents (HSEs) can be used as a valuable tool to study pigmentation in vitro, providing in-depth analysis and characterization of molecular mechanisms associated with melanogenesis. Here, we describe a method to both visualize and quantify basal melanocytes within the epidermis of a pigmented HSE. By using an image analysis pipeline, we can determine how melanocyte density changes in response to treatment and how melanogenic activity is altered using immunofluorescent staining techniques.

Indexed as

MelanocytesSkinEpidermisFluorescent Antibody TechniqueHumansMelaninsMelanogenesisSkin PigmentationMelaninsEpidermal WholemountHuman skin equivalentsImmunofluorescenceMelanocyteMelanogenesisPigmentation

Identifiers

PMID41196494

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.