Evidence map›Paper›PMID 41195825›Full record

ArticleBiology of the cell2025

Sestrin2 is Induced Upon Cellular Stress but Has No Effect on Myotube Size or Amino Acid Sensing in C2C12 Myotubes.

Jiani Qian, Stephanie D Gagnon, Vladimir Belhac, Carl J Hulston, Neil R W Martin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of the cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jiani QianSchool of Sport, Exercise and Health Sciences, Loughborough University, Leicestershire, UK.
Stephanie D GagnonSchool of Sport, Exercise and Health Sciences, Loughborough University, Leicestershire, UK.
Vladimir BelhacSchool of Sport, Exercise and Health Sciences, Loughborough University, Leicestershire, UK.
Carl J HulstonSchool of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham, UK.
Neil R W MartinSchool of Sport, Exercise and Health Sciences, Loughborough University, Leicestershire, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sestrins are a stress-inducible family of proteins that function in cell survival and nutrient sensing through their regulation of mTORC1. Muscle wasting is associated with cellular stress, but to date, there is limited in vitro research investigating sestrins in skeletal muscle cells. Here we use C2C12 myotubes to understand how sestrin proteins (sestrin 1-3) are regulated by different forms of cellular stress linked to muscle wasting conditions. Furthermore, since sestrin2 is a well-characterised protein but is lowly expressed in muscle tissue in the absence of stress, we also aimed to determine if silencing this protein impacted parameters of muscle growth or nutrient sensing by mTORC1 under basal conditions. Incubating C2C12 myotubes with the endoplasmic reticulum (ER) stress-inducing agent tunicamycin, or a high concentration (1000 µM) of hydrogen peroxide (H

Indexed as

Amino AcidsMuscle Fibers, SkeletalSestrinsActivating Transcription Factor 4AnimalsCell LineEndoplasmic Reticulum StressHydrogen PeroxideMechanistic Target of Rapamycin Complex 1MiceTranscription Factor CHOPActivating Transcription Factor 4Amino AcidsAtf4 protein, mouseHydrogen PeroxideMechanistic Target of Rapamycin Complex 1Sesn2 protein, mouseSestrinsTranscription Factor CHOPER stressmTORC1myotubesnutrient sensingskeletal muscle

Identifiers

PMID41195825
PMCPMC12590934

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.