ArticleeLife2025
Longitudinal awake imaging of mouse deep brain microvasculature with super-resolution ultrasound localization microscopy.
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Differential aperture modulation enables high-fidelity 3D photoacoustic imaging.Photoacoustics · 2026Article
- Simultaneous mesoscopic imaging of neural activity and hemodynamics reveals anesthetic modulation of neurovascular responses.Photoacoustics · 2026Article
- Longitudinal Awake Mouse Brain Imaging Using Functional Ultrasound and Functional Ultrasound Localization Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Preclinical in-vivo cerebrovascular imaging in rodent models: Methods and applications in Alzheimer's disease.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Review
- Microbubble Track-Based Functional Ultrasound Localization Microscopy in Awake Mice.IEEE transactions on medical imaging · 2026Article
- Noninvasive whole-brain imaging of glymphatic dynamics.Science advances · 2026Article
- Microbubble Backscattering Intensity Improves the Sensitivity of Three-Dimensional (3-D) Functional Ultrasound Localization Microscopy (fULM).IEEE transactions on medical imaging · 2026Article
- Noninvasive photoacoustic computed mesoscopy for longitudinal brain imaging.Science advances · 2026Article
- Regional brain and cerebrovasculature morphology during normative aging in male and female C57BL/6N mice.Frontiers in aging neuroscience · 2026Article
- Article
- Microbubble Backscattering Intensity Improves the Sensitivity of Three-dimensional (3D) Functional Ultrasound Localization Microscopy (fULM).bioRxiv : the preprint server for biology · 2025Article
- 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline.Communications engineering · 2025Article
- Comparison of high-frequency ultrasound transducers for microvascular localization microscopy in the mouse brain.Imaging neuroscience (Cambridge, Mass.) · 2025Article
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7 authors.
Funding
Abstract
Ultrasound localization microscopy (ULM) is an emerging imaging modality that resolves microvasculature in deep tissues with high spatial resolution. However, existing preclinical ULM applications are largely constrained to anesthetized animals, introducing confounding vascular effects such as vasodilation and altered hemodynamics. As such, ULM quantifications (e.g., vessel diameter, density, and flow velocity) may be confounded by the use of anesthesia, undermining the usefulness of ULM in practice. Here, we introduce a method to address this limitation and achieve ULM imaging in awake mouse brain. Pupillary monitoring was used to support the presence of the awake state during ULM imaging. Vasodilation induced by isoflurane was observed by ULM. Upon recovery to the awake state, reductions in vessel density and flow velocity were observed across different brain regions. In the cortex, the effects induced by isoflurane are more pronounced on venous flow than on arterial flow. In addition, serial in vivo imaging of the same animal brain at weekly intervals demonstrated the highly robust longitudinal imaging capability of the proposed technique. The consistency was further verified through quantitative analysis on individual vessels, cortical regions of arteries and veins, and subcortical regions. This study demonstrates longitudinal ULM imaging in the awake mouse brain, which is crucial for many ULM brain applications that require awake and behaving animals.
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