Evidence map›Paper›PMID 41194377›Full record

ArticleMicrocirculation (New York, N.Y. : 1994)2025

Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.

Victoria E Sturgess, Nadia K Korovesis, Domingo E Uceda, Ali Citalan-Madrid, Eric V Vu, Katherine Stangis, Françoise Van den Bergh, Salman I Essajee, Binyamin Jacobovitz, Gregory B Sands and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microcirculation (New York, N.Y. : 1994), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Victoria E SturgessDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0000-0002-7413-8657
Nadia K KorovesisDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0009-0001-3727-4115
Domingo E UcedaSection of Vascular Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0000-0001-9916-8898
Ali Citalan-MadridMichigan Neuroscience Institute, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Eric V VuCollege of Literature, Science, and the Arts, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0009-0003-2593-992X
Katherine StangisMichigan Neuroscience Institute, University of Michigan Medical School, Ann Arbor, Michigan, USA.ORCID 0009-0009-6071-0366
Françoise Van den BerghDepartment of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0000-0003-4025-6863
Salman I EssajeeDepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas, USA.ORCID 0009-0009-0563-3636
Binyamin JacobovitzMicroscopy Core, Biomedical Research Core Facilities, University of Michigan, Ann Arbor, Michigan, USA.
Gregory B SandsAuckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.ORCID 0000-0002-7875-2099
Daniel A LawrenceDepartment of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA.ORCID 0000-0003-3126-1935
Johnathan D TuneDepartment of Physiology and Anatomy, University of North Texas Health Science Center, Fort Worth, Texas, USA.ORCID 0000-0003-2959-0801
Daniel A BeardDepartment of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0000-0003-0974-2353
Geoffrey G MurphyMichigan Neuroscience Institute, University of Michigan Medical School, Ann Arbor, Michigan, USA.ORCID 0000-0003-3691-1722
C Alberto FigueroaDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.ORCID 0000-0002-3934-6506

Funding

Disentangling the Mechanisms of Coronary Blood Flow Regulation through Multi-scale ModelingR01HL158723 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BEARD, DANIEL A, FIGUEROA, CARLOS ALBERTO · 2022 to 2025
$2.7M
Systems and Integrative Biology Training ProgramT32GM150581 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DANIEL A BEARD · 2023 to 2026
$1.2M
NHLBI NIH HHS R01 HL158723NIGMS NIH HHS T32 GM150581NIH HHS R01-AG074552NIH HHS R01-AG081981NIH HHS R01-HL158723NIH HHS R01HL158723-03S1NIH HHS T32-GM150581
6 · The paper itself

Abstract

objectiveRecent innovations in optical microscopy and tissue preparation permit 3D visualization of complex microvascular networks. Tissue clearing techniques improve light penetration and extend imaging depth. Typically, perfusion-based approaches are used for vascular labeling and tissue clearing. However, immersion-based methodologies provide enhanced practicality when processing tissues from larger animal models.

methodsWe present an immersion-based microvascular labeling and tissue clearing protocol for myocardial tissues using tomato lectin and CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis), demonstrating success for imaging depths of up to 150 μm. This protocol optimized the delipidation and quenching stages for rat and pig myocardial tissues. Image quality was assessed using an automated analysis of signal-to-noise ratios (SNR) and average z-slice intensities.

resultsOptimal image quality was obtained with 24-h CUBIC Reagent I incubation times. Quenching agents TrueVIEW, Glycine, and Trypan Blue did not significantly impact SNR values. TrueBlack and Sudan Black B showed trends of reduced imaging depth compared to controls without quencher incubation. Overall, rat myocardial tissues had higher SNRs than pig tissue samples.

conclusionsThis protocol provides a rigorous foundation for the optimization of immersion-based approaches for myocardial tissue clearing. Future studies will quantify anatomical and topological coronary microvascular to compare disease states.

Indexed as

MyocardiumOptical ImagingAnimalsImaging, Three-DimensionalRatsSwineconfocal microscopyCUBICdelipidationimaging depthmyocardial microvasculature

Identifiers

PMID41194377
PMCPMC12589860

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.