TRIM7 suppresses transmissible gastroenteritis virus replication by targeting the degradation of N protein and activating RIG-I-mediated type I IFN antiviral response.

Weiyun Qin, Yunxiao Xie, Haijiang Liang, Zuolin Wu, Songbai Yang, Xiaolong Zhou, Ayong Zhao

Abstract read

In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Weiyun QinKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China.ORCID http://orcid.org/0000-0002-6298-8785

Yunxiao XieCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Haijiang LiangKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China.

Zuolin WuKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China.

Songbai YangKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China.

Xiaolong ZhouKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China.

Ayong ZhaoKey Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, 310000, China. zay503@zafu.edu.cn.ORCID http://orcid.org/0000-0001-8789-7858

Funding

Natural Science Foundation of Zhejiang Province in China ZCLQN25C1702Research Startup Foundation of Zhejiang Agriculture and Forestry University 2024LFR035

6 · The paper itself

Abstract

Transmissible gastroenteritis virus (TGEV) is a major enteric pathogen causing severe diarrhea in swine, with substantial implications for the biosecurity of intensive pig production worldwide. The E3 ubiquitin ligase TRIM7 has emerged as a key regulator of host antiviral immunity, yet its role in TGEV infection remains elusive. Here, we report that TRIM7 is abundantly expressed in porcine intestinal tissue and significantly upregulated upon TGEV challenge in IPEC-J2 cells. Functional studies demonstrated that TRIM7 restricts TGEV replication in IPEC-J2 cells through two distinct mechanisms. Firstly, molecular interaction and co-immunoprecipitation assays revealed that TRIM7 mediates ubiquitin-dependent degradation of the TGEV nucleocapsid (N) protein. Secondly, RNA-seq analysis combined with RIG-I signaling modulation experiments established that TRIM7 enhances RIG-I-mediated type I interferon (IFN) signaling to suppress viral propagation. These findings uncover a dual antiviral role for TRIM7 in countering TGEV infection, highlighting its potential as a therapeutic target for TGEV control.

Indexed as

DEAD Box Protein 58Gastroenteritis, Transmissible, of SwineInterferon Type INucleocapsid ProteinsTransmissible gastroenteritis virusTripartite Motif ProteinsUbiquitin-Protein LigasesVirus ReplicationAnimalsCell LineSwineDEAD Box Protein 58Interferon Type INucleocapsid ProteinsTripartite Motif ProteinsUbiquitin-Protein LigasesRIG-I signaling pathwayTransmissible gastroenteritis virusTRIM7type I interferonubiquitination

Identifiers

PMID41194212

DOI10.1186/s13567-025-01610-z

PMCPMC12587667

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.