Evidence map›Paper›PMID 41194159›Full record

ArticleBMC pulmonary medicine2025

Genomic and phenotypic characterization of respiratory pathogens from CF pediatric patients from Dnipro, Ukraine.

Dmytro Stepanskyi, Oksana Ishchenko, Patrick McGann, Yuliia Lapa, Iryna Koshova

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Article in BMC pulmonary medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Dmytro StepanskyiDepartment of Microbiology, Virology, Immunology, Epidemiology and Biomedical Physics and Informatics, Dnipro State Medical University, Dnipro, Ukraine. dstepanskiy@gmail.com.
Oksana IshchenkoDepartment of Microbiology, Virology, Immunology, Epidemiology and Biomedical Physics and Informatics, Dnipro State Medical University, Dnipro, Ukraine.
Patrick McGannMultidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Yuliia LapaDepartment of Microbiology, Virology, Immunology, Epidemiology and Biomedical Physics and Informatics, Dnipro State Medical University, Dnipro, Ukraine.
Iryna KoshovaDepartment of Microbiology, Virology, Immunology, Epidemiology and Biomedical Physics and Informatics, Dnipro State Medical University, Dnipro, Ukraine.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChronic infections in cystic fibrosis (CF) patients are often driven by adaptive and drug-resistant bacterial lineages. Understanding sequence types (STs) and phenotypic adaptations such as small colony variants (SCVs) can guide more effective clinical management.

methodsSeventeen bacterial isolates from respiratory specimens of 12 pediatric CF patients (Dnipro, Ukraine, Sept 2022-Sept 2024) were characterized via culture-based diagnostics and whole genome sequencing (WGS). Multi-Locus Sequence Typing (MLST) was applied to determine sequence types, while phenotypic traits, including SCV morphology, were recorded and antimicrobial resistance (AMR) genes profiled.

resultsThe collection included Staphylococcus aureus (n = 8), Pseudomonas aeruginosa (n = 5), Serratia marcescens (n = 2), Enterobacter hormaechei (n = 1), and Klebsiella pneumoniae (n = 1). SCV phenotypes were observed in 2 S. aureus ST398 isolates. P. aeruginosa strains belonged to diverse STs (ST644, ST2967, ST1228, ST242). The hypervirulent K. pneumoniae ST23-KL1 isolate harbored siderophores (iucA, ybt) and peg-344. All S. aureus ST398 isolates exhibited reduced growth rate and dense colony morphology.

conclusionThis study highlights the genetic and phenotypic complexity of respiratory pathogens in Ukrainian CF children, emphasizing the emergence of ST398 SCVs and the introduction of hypervirulent K. pneumoniae lineages. The findings call for regular genomic surveillance in Ukrainian CF cohorts.

Indexed as

Cystic FibrosisRespiratory Tract InfectionsAdolescentAnti-Bacterial AgentsChildChild, PreschoolDrug Resistance, BacterialEnterobacterFemaleGenomicsHumansInfantKlebsiella pneumoniaeMaleMicrobial Sensitivity TestsMultilocus Sequence TypingAnti-Bacterial AgentsCystic fibrosisGenomic characterizationPseudomonas aeruginosaSmall-colony variants Staphylococcus aureusUkraine

Identifiers

PMID41194159
PMCPMC12587554

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