Evidence map›Paper›PMID 41193967›Full record

ArticleBMC plant biology2025

Assembling the chromosome-level genome of Arctium Lappa L. provides new insights into carotenoid biosynthesis.

Xingxing Feng, Kuanqiang Tang, Yanxing Wang, Na Zhang, Shuo Li, Liang Luo, Enqi Liu, Anhui Chen, Shuai Wang, Tingguo Kang and 3 more

Abstract read
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xingxing FengSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Kuanqiang TangCollege of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, 161006, China.
Yanxing WangSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Na ZhangSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Shuo LiSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Liang LuoSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Enqi LiuSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Anhui ChenSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Shuai WangSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China.
Tingguo KangSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116600, China.
Deqiang DouSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116600, China.
Chuanyin HuXuzhou Tian Ma Jing An Food., Ltd, Xuzhou, 221600, China.
Yuwei DongSchool of Food and Bio-engineering, Xuzhou University of Technology, Xuzhou, 221018, China. dongyuwei66@163.com.

Funding

Jiangsu Province Industry-University-Research Cooperation Project BY20231322
6 · The paper itself

Abstract

backgroundArctium lappa L. (A. lappa), a plant of significant economic importance, is cultivated worldwide for its applications in food and medicine, presenting a compelling opportunity for genetic research within the Asteraceae family. Despite the importance of A. lappa genetic resources, the genome of the Peixian yellow A. lappa cultivar has not yet been sequenced.

resultsThis study presents a chromosome-level genome of the Peixian yellow A. lappa, the genome consists of a comprehensive 1.69 Gb reference and a scaffold N50 of 91.02 Mb. By utilizing Hi-C data, we anchored 99.12% of the assembled sequences onto 18 chromosomes and identified 40,665 protein-coding genes. The genome is predominantly composed of repetitive sequences, which constitute 68.32%, with long terminal repeats (LTRs) accounting for 65.66% of the total. A comparative genomic analysis with the Gansu A. lappa genome revealed significant genetic variations, including 2,972,809 single nucleotide polymorphisms (SNPs), 608,644 insertions/deletions (INDELs), and 11,800 structural variations (SVs). Furthermore, transcriptomic analyses comparing Peixian yellow A. lappa and Liu chuan li xiang A. lappa have shed light on the molecular mechanisms underlying carotenoid biosynthesis in Peixian yellow A. lappa. A total of 16 differentially expressed genes (DEGs) were specifically associated with the carotenoid biosynthesis pathway. Among these, several genes, including PSY1, PDS, BCH2, VDE, NCED1, and NCED2, were downregulated in Peixian yellow A. lappa. In contrast, other genes such as DXS, IDI1, CCD8B, CCS, and CCD4 were upregulated. Additionally, several transcription factors linked to carotenoid biosynthesis were identified, including MYC2, WRK35, and HY5. Most of these transcription factors were downregulated in Peixian yellow A. lappa, with the exception of Arcla.08g00830, which encodes MYC2.

conclusionThis high-quality genomic resource is poised to enhance genome-informed breeding strategies and improve A. lappa quality, offering valuable insights for breeding programs aimed at enhancing desirable traits in A. lappa.

Indexed as

ArctiumCarotenoidsChromosomes, PlantGenome, PlantPolymorphism, Single NucleotideCarotenoidsA. lappaCarotenoid biosynthesisGenome assemblyHiFi

Identifiers

PMID41193967
PMCPMC12590599

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.