Evidence map›Paper›PMID 41193640›Full record

ArticleThe EMBO journal2025

Structural insights into the atypical filament assembly of pyrin domain-containing IFI16.

Archit Garg, Ewa Niedzialkowska, Jeffrey J Zhou, Jasper Moh, Edward H Egelman, Jungsan Sohn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Archit GargDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.ORCID http://orcid.org/0000-0002-5931-2522
Ewa NiedzialkowskaDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, 22098, USA.
Jeffrey J ZhouDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.ORCID http://orcid.org/0009-0005-9083-4897
Jasper MohDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Edward H EgelmanDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, 22098, USA.ORCID http://orcid.org/0000-0003-4844-5212
Jungsan SohnDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA. jsohn@jhmi.edu.ORCID http://orcid.org/0000-0002-9570-2544

Funding

Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic ResolutionR35GM122510 · NIGMS · UNIVERSITY OF VIRGINIA · PI EDWARD H. EGELMAN · 2017 to 2026
$7.3M
Mechanistic Studies of cytosolic double-stranded DNA sensing pathways.R35GM145363 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI JUNGSAN SOHN · 2022 to 2026
$2.3M
HHS | National Institutes of Health (NIH) R35GM122510HHS | National Institutes of Health (NIH) R35GM145363Johns Hopkins University (JHU) Accelerator AwardNational Science Foundation (NSF) MCB1845003National Science Foundation (NSF) MCB2501395NIGMS NIH HHS R35 GM122510NIGMS NIH HHS R35 GM145363
6 · The paper itself

Abstract

In response to various intracellular stress or damage-associated signals, inflammasomes can be activated and trigger a pyroptotic cell death process through the sequential assembly of structurally compatible and interacting filamentous oligomers involving the pyrin domains (PYD) of important inflammasome components. The PYD-containing interferon-inducible protein 16 (IFI16) has been suggested as a viral DNA sensor that can induce inflammasome formation, but it also has other inflammasome-independent functions, including interferon production. Here, the cryo-EM structure of the filament assembled by the PYD of human IFI16 reveals a helical architecture distinct from inflammasome PYD filaments. In silico interface energy calculations suggest that the helical architecture of the IFI16

Indexed as

InflammasomesNuclear ProteinsPhosphoproteinsPyrin DomainCryoelectron MicroscopyHumansModels, MolecularIFI16 protein, humanInflammasomesNuclear ProteinsPhosphoproteinsCryo-EMInflammasomesInnate Immune SystemInterferon-inducible Protein 16Pyrin Domain

Identifiers

PMID41193640
PMCPMC12705702

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.