ArticleBMC biology2025
Function and defense mechanisms of wheat 14-3-3 protein TaGF14a in stripe rust resistance.
Article in BMC biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- 14-3-3 proteins: Central hubs in plant development and stress adaptation.Journal of integrative plant biology · 2026Review
- Integrated Transcriptomic and Metabolomic Analyses Reveal the Mechanism by Which 5-Methoxyindole Enhances Sesquiterpenoids Production inPlants (Basel, Switzerland) · 2026Article
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5 authors.
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Abstract
backgroundAs critical regulatory elements in plant signaling transduction, 14-3-3 proteins orchestrate defense responses against biotic and abiotic stresses. This study investigates the functional role of TaGF14a in wheat's immune response to Puccinia striiformis f. sp. tritici (Pst), providing novel genetic insights for stripe rust resistance breeding.
resultsPhylogenetic analysis revealed a close evolutionary relationship between TaGF14a and rice OsGF14a. Subcellular localization studies demonstrated that the protein was located in the in the nucleus, cytoplasm, and plasma membrane. Pathogen challenge experiments showed significant induction of TaGF14a expression following Pst inoculation. Virus-induced gene silencing (VIGS) technology was used to silence TaGF14a, which resulted in expanded necrotic lesions and compromised fungal containment during incompatible interactions. Concurrently, we observed downregulation of pathogenesis-related genes (TaPR1, TaPR3) and altered expression patterns in reactive oxygen species (ROS) metabolism genes (TaCAT, TaSOD). Protein interaction assays identified novel associations between TaGF14a and key defense components: xylanase inhibitor and E3 ubiquitin ligase RGLG2, suggesting coordinated regulation of cell wall reinforcement and proteasomal degradation pathways. Furthermore, TaGF14a exhibited dual stress responsiveness, showing 3.3-fold induction under methyl jasmonate treatment and 3-fold increase under cold stress (4 °C).
conclusionsThis comprehensive analysis advances our understanding of 14-3-3 proteins in cereal-pathogen interactions and provides molecular targets for durable disease resistance strategies.
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