ArticleCell reports methods2025
Targeted long-read methylation analysis using hybridization capture suitable for clinical specimens.
Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Structural variant discovery and diagnostic impact in rare diseases from short-read and long-read sequencing.medRxiv : the preprint server for health sciences · 2026Article
- Spatial gene expression analysis reveals drivers of extremely early lymph node metastasis in breast cancer.NPJ breast cancer · 2026Article
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Authors and funding
17 authors.
Funding
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Abstract
To detect precise DNA methylation patterns in long-read DNA sequencing analysis, an efficient target enrichment method is needed. In this study, we established t-nanoEM, a practical method that integrates a hybridization-based capture step into a long-read enzymatic methyl (EM)-seq library for nanopore sequencing. We achieved a high sequencing coverage of up to ×570 at 5 kb N50 in length. We applied this method to the long-read methylation analysis of cancers. Using breast cancer as an example, we demonstrated that the signature changes in DNA methylation occurring in local cell populations could be displayed in a haplotype-aware manner. In lung cancer, the spatial diversity in gene expression as detected by the spatial expression profiling analysis may be associated with changes in DNA methylation.
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